Analysis of Expression of Nitric Oxide Syntheses and Their Functional Role in Human Urothelium - Full Text View

Sponsors and Collaborators:	Copenhagen University Hospital at Herlev Roskilde County Hospital
Information provided by:	Copenhagen University Hospital at Herlev
ClinicalTrials.gov Identifier:	NCT00253006

Purpose

Painful bladder syndrome (PBS)/interstitial cystitis (IC) may be due to the actions of nitric oxide (NO) in the bladder tissue. NO is a gaseous substance with a very short half-life, synthesized by a group of NO-synthase-enzymes in many tissues.

The goal of this study is to illuminate bladder tissue production of NO in individuals with PBS and healthy individuals, by quantification of NO and NO-enzyme expression by different molecular biological methods.

Condition	Intervention
Interstitial Cystitis Bladder Diseases Urinary Incontinence	Procedure: Tissue examination for protein- and mRNA-expression of NO

MedlinePlus related topics: Bladder Diseases Interstitial Cystitis Urinary Incontinence

Drug Information available for: Nitric oxide

U.S. FDA Resources

Study Type:	Observational
Study Design:	Natural History, Cross-Sectional, Defined Population, Retrospective/Prospective Study
Official Title:	A Quantitative Analysis of Protein- and mRNA-Expression of Nitric Oxide Syntheses and Their Functional Role in Human Urothelium

Further study details as provided by Copenhagen University Hospital at Herlev:

Estimated Enrollment:	60
Study Start Date:	January 2006
Estimated Study Completion Date:	January 2008

Eligibility

Ages Eligible for Study:	18 Years and older
Genders Eligible for Study:	Both
Accepts Healthy Volunteers:	No

Criteria

Inclusion Criteria:

PBS/IC according to International Continence Society (ICS)
Cystoscopy with bladder distension and deep biopsies within last 5 years
Cystometry within last 5 years
Normal urine culture within last month

Inclusion Criteria (for control group):

Benign non-invasive bladder disease
No PBS/IC
Normal urine culture
No functional bladder disease of known nature (ex. infravesical obstruction)

Exclusion Criteria:

Age under 18 years
Inability to understand instructions
Pregnancy and lactation

Contacts and Locations

Please refer to this study by its ClinicalTrials.gov identifier: NCT00253006

Locations

Denmark
Copenhagen University Hospital at Herlev
Herlev, Denmark, DK- 2730

Sponsors and Collaborators

Copenhagen University Hospital at Herlev

Roskilde County Hospital

Investigators

Study Director:

Pierre Bouchelouche, MD

Roskilde County University Hospital at Koege, Dept. of Clinical Biochemistry

More Information

Publications:

Birder LA, Wolf-Johnston A, Buffington CA, Roppolo JR, de Groat WC, Kanai AJ. Altered inducible nitric oxide synthase expression and nitric oxide production in the bladder of cats with feline interstitial cystitis. J Urol. 2005 Feb;173(2):625-9.

Bouchelouche K, Nordling J. Recent developments in the management of interstitial cystitis. Curr Opin Urol. 2003 Jul;13(4):309-13. Review.

Logadottir YR, Ehren I, Fall M, Wiklund NP, Peeker R, Hanno PM. Intravesical nitric oxide production discriminates between classic and nonulcer interstitial cystitis. J Urol. 2004 Mar;171(3):1148-50; discussion 50-1.

Study ID Numbers:	UA_H_Ka-05104-m
Study First Received:	November 11, 2005
Last Updated:	October 6, 2006
ClinicalTrials.gov Identifier:	NCT00253006
Health Authority:	Denmark: Danish Medicines Agency

Keywords provided by Copenhagen University Hospital at Herlev:

NO
mRNA-expression
pathophysiology
painful bladder syndrome

Study placed in the following topic categories:

Nitric Oxide
Signs and Symptoms
Cystitis, Interstitial
Cystocele
Urologic Diseases

Urination Disorders
Urinary Bladder Diseases
Cystitis
Urinary Incontinence
Pain

Additional relevant MeSH terms:

Respiratory System Agents
Vasodilator Agents
Neurotransmitter Agents
Antioxidants
Molecular Mechanisms of Pharmacological Action
Physiological Effects of Drugs
Anti-Asthmatic Agents
Cardiovascular Agents
Protective Agents

Pharmacologic Actions
Urological Manifestations
Autonomic Agents
Therapeutic Uses
Free Radical Scavengers
Endothelium-Dependent Relaxing Factors
Peripheral Nervous System Agents
Bronchodilator Agents

ClinicalTrials.gov processed this record on January 14, 2009