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Sponsors and Collaborators: |
University of Connecticut Health Center EMD Serono |
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Information provided by: | University of Connecticut |
ClinicalTrials.gov Identifier: | NCT00602966 |
Oocyte cryopreservation has been studied for many years without much success in refining a method that has consistent, reliable results in producing viable embryos and clinical pregnancies. In 1986 the first baby was born from an embryo created from a frozen oocyte; however, since then there have been less than 150 births from frozen eggs. To date, there are no reportable adverse outcomes in the children born from frozen oocytes. The research continues to look at different methods of oocyte cryopreservation. Many smaller studies have been conducted with some success but larger clinical trials are needed to replicate these findings. The conventional cryopreservation technique has been slow cooling with differing methods of freezing; however, vitrification is now being researched as the potential cryopreserving method that holds some promise for the future.
Our hypothesis is the use of vitrification (quick freezing) to cryopreserve oocytes in patients undergoing in-vitro fertilization will be more successful than slow freezing in oocyte survival, fertilization rate with ICSI and subsequent embryo development, implantation rate and pregnancy rate.
Condition |
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Infertility |
Study Type: | Observational |
Study Design: | Case Control, Prospective |
Official Title: | Oocyte Cryopreservation: Comparison of Slow Cooling Versus Vitrification Techniques on Oocyte Survival, Fertilization, and Embryo Development |
Estimated Enrollment: | 30 |
Study Start Date: | July 2006 |
Estimated Study Completion Date: | January 2010 |
Estimated Primary Completion Date: | October 2009 (Final data collection date for primary outcome measure) |
Groups/Cohorts |
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Slow Freeze |
Vitrification |
Cryopreservation of oocytes is desirable because it: 1) would allow infertility patients to store excess oocytes instead of embryos, eliminating some of the ethical and religious concerns that accompany embryo storage; 2) permit storage of donor oocytes to avoid donor-recipient synchronization difficulties; and 3) can help women who may face sterilization due to chemotherapy or radiation. Oocyte cryopreservation is therefore gaining in popularity as an option for infertility treatment as well as fertility preservation.
Oocyte cryopreservation using conventional slow-cooling methods has not had much success; however more recent results have provided more optimism (Boldt et al., 2003; Porcu et al., 1997; 2000; 2002; Yang et al., 1998; 1999; 2002; Winslow et al., 2001). Vitrification has also been employed (Hong et al., 1999; Kuleshova et al., 1999; Yoon et al., 2000, 2003; Chung et al 2000; Wu et al., 2001: Kuwayama et al., 2005) with increased oocyte survival rate and live births. Vitrification is performed by suspending the oocytes in a solution containing a high concentration of cryoprotectants and then plunging them directly into liquid nitrogen (Rall and Fahy, 1985). The advantage of this technique is to prevent the formation of ice crystals within the oocyte. However the toxic effect of the high concentration of the cryoprotectant media has been a concern. New vitrification techniques which attempt to accelerate the cooling rate by decreasing the cryosolution volume and concentration, may reduce the potential toxicity. In addition, a more rapid cooling rate results in reduced chilling injury (Vajta et al., 1998).
Ages Eligible for Study: | 21 Years to 36 Years |
Genders Eligible for Study: | Female |
Accepts Healthy Volunteers: | Yes |
Sampling Method: | Probability Sample |
The Center for Advanced Reproductive Services patient population
Inclusion Criteria:
Exclusion Criteria:
United States, Connecticut | |
The Center for Advanced Reproductive Services | |
Farmington, Connecticut, United States, 06030-6224 |
Principal Investigator: | Claudio Benadiva, MD, HCLD | The Center for Advanced Reproductive Services, P.C. |
Responsible Party: | The Center for Advanced Reproductive Services ( Dr. Claudio Benadiva, MD, HCLD ) |
Study ID Numbers: | 06-336-2, 26525 |
Study First Received: | January 15, 2008 |
Last Updated: | October 1, 2008 |
ClinicalTrials.gov Identifier: | NCT00602966 |
Health Authority: | United States: Institutional Review Board |
Oocyte cryopreservation slow freeze vitrification |
Genital Diseases, Female Infertility Genital Diseases, Male |