Enzymatically-Active RNA-Dependent RNA Polymerase From a Human Norovirus (Calicivirus)
Description of Invention:
The noroviruses (formerly known as “Norwalk-like viruses”) are associated with gastroenteritis outbreaks, affecting large numbers of individuals each year. Emerging data are supporting their increasing recognition as important agents of diarrhea-related morbidity and mortality. The frequency with which noroviruses are associated with gastroenteritis as “food and water-borne pathogens” has led to the inclusion of caliciviruses as Category B Bioterrorism Agents/Diseases. Because the noroviruses cannot be propagated by any means in the laboratory, an important strategy in their study is to development of molecular biology-based tools and replication systems. This invention reports the isolation of the first recombinant, enzymatically-active proteinase and RNA dependent RNA polymerase (RdRp) complex for a human norovirus. This enzyme should facilitate studies aimed at developing therapeutic drugs for norovirus disease.
Inventors:
Gael M. Belliot (NIAID) Stanislav V. Sosnovtsev (NIAID) Kyeong-Ok Chang (NIAID) Kim Y. Green (NIAID)
Patent Status:
DHHS Reference No. E-283-2003/0 -- Research Material
Related Technologies:
DHHS Reference No. E-198-2003/0 -- Research Material
Construction of Recombinant Baculoviruses Carrying the Gene Encoding the Major Capsid Protein, VP1, from Calicivirus Strains (including Norovirus Strains Toronto, Hawaii, Desert Shield, Snow Mountain, and MD145-12)
DHHS Reference No. E-212-2003/0 – Research Material
Full-Length cDNA Clone Representing the Consensus Sequence of the RNA Genome of a Human Norovirus (strain MD145-12) that Encodes Biologically Active Proteins
DHHS Reference No. E-214-2003/0 -- Research Material
Construction of an Infectious Full-Length cDNA Clone of the Porcine Enteric Calicivirus RNA Genome
Relevant Publication: The materials are further described in L Wei et al., "Proteinase-polymerase precursor as the active form of feline calicivirus RNA-dependent RNA polymerase," J. Virol. 2001 Feb;75(3):1211-1219. [PubMed abs]
Licensing Status: The materials embodied in this invention are available nonexclusively through a biological materials license.
Collaborative Research Opportunity:
The Laboratory of Infectious Diseases, NIAID, NIH, is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize an active human norovirus proteinase-polymerase enzyme. Please contact Kim Y. Green at kgreen@niaid.nih.gov for more information.
For Additional Information Please Contact: Peter A. Soukas J.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-4646
Email: soukasp@mail.nih.gov
Fax: (301) 402-0220
