High Speed Parallel Molecular Nucleic Acid Sequencing
Description of Invention:
Available for licensing and commercial development is a new system, methods and compositions for DNA sequencing, also known as Two Dye Sequencing (TDS). This invention is based on Fluorescence Resonance Energy Transfer (FRET), a technology increasingly in use for several molecular analysis purposes. In particular, the method consists of:
attachment of engineered DNA polymerases labeled with a donor fluorophore to the surface (chamber) of a microscope field of view;
addition to the chamber of DNA with an annealed oligonucleotide primer, which is bound by the polymerase;
further addition of four nucleotide triphosphates, each labeled on the base with a different fluorescent acceptor dye;
excitation of the donor fluorophore with light of a wavelength specific for the donor but not for any of the acceptors, resulting in the transfer of the energy associated with the excited state of the donor to the acceptor fluorophore for a given nucleotide, which is then radiated via FRET;
identification of the nucleotides most recently added to the primer by recording the fluorescent spectrum of the individual dye molecules at specific locations in the microscope field, and
converting the sequential spectrum into a DNA sequence for each DNA molecule in the microscope field of view.
Application:
Sequencing of single nucleic acid molecules on a substrate
Patent Status:
DHHS Reference No. E-033-1999/0 --
U.S. Patent No. 6,982,146 issued 03 Jan 2006
U.S. Patent Application No. 11/204,367 filed 12 Aug 2005
Licensing Status: Available for co-exclusive licensing.
Portfolios: Devices/Instrumentation
Devices/Instrumentation-Diagnostics-Methods Devices/Instrumentation-Research Tools and Materials-Methods of Using Research Tools Devices/Instrumentation-Diagnostics Devices/Instrumentation-Research Materials
For Additional Information Please Contact: Cristina Thalhammer-Reyero PhD MBA
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-4507
Email: thalhamc@mail.nih.gov
Fax: (301) 402-0220
