Layered Electrophoresis Scan: A Method For High Throughput Molecular Fingerprinting Of Tissue And Cell Samples
Description of Invention:
Layered expression scanning is a technique which combines tissue and/or cell samples with a high-throughput array approach to provide a simple and rapid method for comprehensive molecular analysis. The method works by placing a biological sample (tissue section, or dissected cell populations, or lysates from cells) adjacent to a set of capture layers, each containing an individual hybridization molecule (antibody or DNA sequence). The specimen(s) is transferred through the membranes and, importantly, the overall two-dimensional architecture and histological relationships within the sample(s) are maintained. As the proteins and nucleic acids are transferred each target molecule specifically hybridizes to the membrane containing its antibody or complementary DNA sequence. After hybridization each of the membranes are analyzed, providing a measurement of the level of expression of each targeted molecule in all of the cell types present in the sample.
