Pigment Epithelium Derived Factor: Characterization, Genomic Organization and Sequence of PEDF Gene
Description of Invention:
Retinal pigment epithelium (RPE) cells secrete a unique 50 kD protein. This protein, pigment epithelium-derived factor (PEDF), contains trophic activity in both the induction of neuronal cell differentiation as well as the survival of the mature neuron. This invention provides the complete genomic sequence of PEDF. It also discloses a truncated version of PEDF referred to as rPEDF, equivalent proteins, vectors comprising the nucleic acids, host cells into which the vectors have been introduced as well as a recombinant method for producing PEDF.
Potential Area of Application:
research reagent
pharmaceutical composition
Main Advantage of Invention:
recombinant method
production of large quantities
Inventors:
G Chader (NEI) SP Becerra (NEI) J Tombran-Tink (NEI) F Steele (NEI) I Rodriguez (NEI) et al.
Patent Status:
DHHS Reference No. E-185-1992/3 --
U.S. Patent 6,319,687 issued 20 Nov 2001
U.S. Patent Application No. 09/630,629 filed 01 Aug 2000
DHHS Reference No. E-185-1992/4 --
PCT/US95/07201 filed 06 Jun 1995, published under WO 95/33480 on 14 Dec 1995
Related Technologies: DHHS Reference No. E-185-1992/1 --
U.S. Patent 5,840,686 issued 23 Nov 1998, entitled "Pigment Epithelium Derived Factor: Characterization of its Novel Biological Activity and Sequences Encoding and Expressing the Protein and Methods of Use," by GJ Chader, SP Becerra, JP Schwartz, T Taniwaki, and Y Sugita
DHHS Reference No. E-185-1992/2 --
U.S. Patent 6,451,763 issued 17 Sep 2002, entitled "Retinal Pigmented Epithelium Derived Neutrophic Factor and Methods of Use," by J Tombran-Tink, G Chader, SP Becerra, I Rodriguez, F Steele, and L Johnson
Relevant Publication:
FR Steele, GJ Chader, LV Johnson, J Tombran-Tink. Pigment epithelium-derived factor: neurotrophic activity and identification as a member of the serine protease inhibitor gene family. Proc Natl Acad Sci USA. 1993 Feb 15;90(4):1526-1530. [PubMed abs]
SP Becerra, I Palmer, A Kumar, F Steele, J Shiloach, V Notario, GJ Chader. Overexpression of fetal human pigment epithelium-derived factor in Escherichia coli: a functionally active neurotrophic factor. J Biol Chem. 1993 Nov 5;268(31):23148-23156. [PubMed abs]
Licensing Status: Available for exclusive or nonexclusive licensing
For Additional Information Please Contact: Susan S. Rucker J.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-4478
Email: ruckersu@mail.nih.gov
Fax: (301) 402-0220
