Description of Invention:
The invention relates to a novel, cost-effective method of detecting HIV antigens, in particular HIV Gag (p24) antigen, in human biological samples. The method relies on using a novel combination of a bead coated with a primary high affinity monoclonal antibody specific for p24 antigen and a secondary antibody conjugated with a fluorescent label that is also specific for p24 antigen. This detection method requires only approximately 50 μl of sample, and is able to detect the presence of HIV p24 antigen over a range of concentrations from 20,000 picograms down to 0.3 picograms with very low intrasample variability. The upper and lower limits of the detection method can be adjusted by altering the components of the assay.
Applications:
Detection of HIV antigens in biological samples
Advantages:
Cost-effective
Minimal amounts of sample required
High sensitivity and dynamic range
Development Status: In vitro data can be provided upon request
For Additional Information Please Contact: Kevin Chang Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: 301/435-5018
Email: changke@mail.nih.gov
Fax: 301/402-0220
