Description of Invention:
Available for licensing and commercial development are methods for identifying soluble proteins in a sample. Identification and characterization of bioactive compounds is a critical step in drug discovery, and there is a need for improved methods for identifying soluble proteins. One method provided, which produces soluble deletion derivatives of a protein, includes the steps of incubating a vector with a nucleic acid sequence encoding the protein, flanked by a first and second site-specific recombination sites, in the presence of one or more transposons with a third and fourth site-specific recombination sites and a transposase protein, to insert the one or more transposons into the vector, followed by transfer to further vectors with additional site-specific recombination sites, which are propagated, isolated, combined and recombined in the presence of a recombinase. A second method is for identifying two or more soluble proteins and includes the steps of expressing two or more vectors with the nucleic acid sequence encoding a soluble protein operatively linked to a promoter in one or more cells, and identifying and quantifying the isolated two or more soluble proteins by mass spectroscopy. The above methods can be used alone or in combination. These methods will enable researchers to identify both individual protein targets of drugs, as well as protein families or protein signaling pathways, thereby enhancing drug development.
Inventors:
James L. Hartley (SAIC/NCI) Dominic Esposito (SAIC/NCI) Kelly Jeanne Stanard (SAIC/NCI)
Patent Status:
DHHS Reference No. E-161-2004/0 --
U.S. Provisional Application No. 60/628,948 filed 19 Nov 2004
PCT Application No. PCT/US2005/19477 filed 03 Jun 2005, which published as WO 2006/055040 on 26 May 2006
Licensing Status: In addition to licensing, the technology is available for further development through collaborative research opportunities with the inventors.
Portfolios: Gene Based Therapies Devices/Instrumentation Cancer
Cancer -Diagnostics-In Vitro-DNA Based Cancer -Diagnostics Devices/Instrumentation-Diagnostics Devices/Instrumentation-Research Materials Gene Based Therapies -Diagnostics
For Additional Information Please Contact: Cristina Thalhammer-Reyero PhD MBA
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-4507
Email: thalhamc@mail.nih.gov
Fax: (301) 402-0220
