Harlan Animal Colonies Health Evaluation Summary: 03/14/08
Contract N01-AG-7-2113
RATS Barrier 202B
| Animal Facility |
Strain |
No.Sex |
Age |
Testing Laboratory/Dates |
| Location: Indianapolis, IN |
F344/NHsd |
2M/2F |
5 months |
University of Missouri |
| Barrier/Building: 202B |
|
1M/1F |
10 months |
MURADIL, Columbia, MO |
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Receipt Date: 1/22/08 |
| Ship Date: 1/21/08 |
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Report Date: 3/4/08 |
Evaluation
| NIA rats submitted for this health monitoring were obtained from NIA-NIH aging colonies maintained in barriers by Harlan. All rats examined were considered to be in a good state of health. |
Serology
| Agent |
Assay |
No. Pos./No. Tested |
Comments |
| Sendai |
MFI |
0/6 |
|
| RCV/SDAV |
MFI |
0/6 |
|
| Parvoviruses |
MFI |
0/6 |
|
| KRV |
MFI |
0/6 |
|
| H-1 |
MFI |
0/6 |
|
| PVM |
MFI |
0/6 |
|
| M. pulmonis |
MFI |
0/6 |
|
| Reo 3 |
MFI |
0/1 |
|
| TMEV GDVII |
MFI |
0/1 |
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| MAD |
MFI |
0/1 |
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| LCM |
MFI |
0/1 |
|
| Hantaan |
MFI |
0/1 |
|
Bacteriology
| Agent |
No. Pos./No. Tested |
Comments |
| Mycoplasma pulmonis |
0/6 |
|
| Salmonella spp. |
0/6 |
Nasopharynx/lung/cecum/uterine cultures |
| Pasturella pneumotropica |
0/6 |
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| Pasturella spp. |
0/6 |
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| Bordetella bronchiseptica |
0/6 |
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| Pseudomonas aeruginosa |
0/6 |
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| Citrobacter rodentium |
0/6 |
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| Corynebaterium kutscheri |
0/6 |
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| Klebsiella pneumoniae |
0/6 |
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| Klebsiella oxytoca |
0/6 |
The bacteria isolated from the nasopharynx and cecum are opportunistic or normal flora of the rat. |
| Staphylococcus aureus |
0/6 |
| Staphylococcus pneumoniae |
0/6 |
|
| Streptococcus sp. Group B beta |
0/6 |
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PCR
| |
No. Pos./No.Tested |
Comments |
| Mycoplasma pulmonis |
0/6 |
|
| Heliobacter hepaticus |
0/6 |
|
Parasitology
| Type and Test |
Results |
Comments |
| Ectoparasites |
No. Pos./No. Tested |
External parasites were not observed in any rat. |
Test: Direct pelage |
0/6 |
| Endoparasites |
No. Pos./No. Tested |
Enteric and urinary bladder parasites were not observed in any rat. |
Test(s): Direct, Gross, and Microscopic (intestines & bladder) |
0/6 |
Pathology
| Gross: |
None Significant |
Overall frequency of lesions in these rats is low. |
| Histologic: |
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| Lung lymphoid hyperplasia |
5/6 |
Lesions are incidental and/or consistent with normal aging process and are not indicative of infection with murine adventitious pathogens. |
| Harderian gland lymphoid infiltrate |
2/6 |
| Nephrocalcinosis |
3/6 |
| Myocardial degeneration |
5/6 |
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A few other incidental lesions were also noted. |
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Availability
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Rats obtained from NIA-NIH aging colonies maintained in a maximum security barrier by Harlan in Indianapolis, Indiana. The combined census of these colonies, at the time of the referenced monitoring, is tabulated below:
|
Retired Breeder - Male (10-26 months of age) |
179 |
|
Virgin - Male (2-32 months of age) |
4,501 |
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Virgin - Female (2-30 months of age)
|
2,702
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Total Census: |
7,382 |
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For information, contact:
Dr. Karla A. Stevens
HARLAN SPRAGUE DAWLEY, INC.
8520 Allison Pointe, #400
Indianapolis, IN 46250
Phone: (317) 806-6060
Fax: (317) 806-6073
Signed by Karla A. Stevens, D.V.M., Principal Investigator
Page last updated Sep 26, 2008
