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Genotyping Analysis

Samples were amplified using PCR-based PowerPlex 1.2 (Promega). This intraspecies human identity test, which examines polymorphic short tandem repeat (STR) DNA regions, uniquely identifies unrelated individuals and cell lines. Most of the above cell lines have been tested by STR analysis and found to be unique. This will assist us in identifying each line and monitoring the purity of the lines over time. (Tests performed by ATCC and Cell Line Genetics.)

BG01 BG02 BG03 ES01 ES02 ES03 ES04 ES05 ES06 SA01 SA02
D5S818 10,12 11,12 13 12,13 11 10,12 10,12 10,12 10,12 12,13 12
D13S317 11,12 11 8,12 8 8,11 9,12 10,11 11 10,11 11,12 9,11,14
D7S820 10,11 12,14 11 11,12 9,11 11,12 9,13 9,13 8,9 8,10 10,12
D16S539 9,11 12 11,13 11 9,12 9 9,11 11,12 12 12,13 12,14
vWA 16,17 18,19 16 17 18,20 17,18 17,19 17,19 17 16,18 14,16
TH01 7,9.3 8,9 6,7 9 7,9 7,9 6,7 6,8 7,10 9.3 9,9.3
Amelogenin XY XY XX XX XX XX XY XY XX XY X
TPOX 8 11 8 8,12 8,12 8 8 8,9 8 8,9 8,9
CSF1PO 10 8,10 12 12 11 12 10 10,11 10,11 10,13 11,12
D8S1179 ND ND ND ND ND ND ND 11,13 ND 13 14,15
FGA ND ND ND ND ND ND ND 23,24 ND 24,26 24,26
Penta E ND ND ND ND ND ND ND 11 ND 9,14 7,12
D18S51 ND ND ND ND ND ND ND 15,20 ND 12,15 15,16
D21S11 ND ND ND ND ND ND ND 29,31 ND 31.2,
32.2		 28,30
D3S1358 ND ND ND ND ND ND ND 14,18 ND 15,17 12,17
Penta D ND ND ND ND ND ND ND 11,12 ND 9,13 8,12
TE03 TE04 TE06 UC01 UC06 WA01 WA07 WA09 WA13 WA14
D5S818 11,12 11,12 12 11,12 11,12 9,11 11,13 11,12 11,13 11,13
D13S317 12,14 12,14 8,11 8,11 9,13 8,11 11,12 9 11,12 11
D7S820 10,11 12,13 8,10 8,10 9,10 8,12 10,11 9,11 10,11 10,11
D16S539 11,12 11,12 9,13 9,10 12 9,13 12,13 12,13 9,11 11,13
vWA 16,17 16,18 16,17 16,18 16 15,17 14,15 17 14,15 15,16
TH01 7,9 7,9 8,9.3 9,10 6,9 9.3 6 9.3 6 6,7
Amelogenin XX X XY XY XX XY XX XX XY XY
TPOX 8 8 10,11 8 8 8,11 8,11 10,11 8,11 11,13
CSF1PO 11,12 12 10,11 11,12 9,12 12,13 12 11 12 11,12
D8S1179 ND 13,14 ND 10,16 14 ND ND ND ND ND
FGA ND 22,23 ND 18,21.2 19,22 ND ND ND ND ND
Penta E ND 5,11 ND 17,20 7,14 ND ND ND ND ND
D18S51 ND 13,15 ND 15,19 14,17 ND ND ND ND ND
D21S11 ND 29,32.2 ND 28,33.2 29 ND ND ND ND ND
D3S1358 ND 16,18 ND 15 17,18 ND ND ND ND ND
Penta D ND 8,13 ND 9,10 10,13 ND ND ND ND ND

 

ND: Not determined.