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Growth Curves

We are calculating the rate of growth of each cell line by counting the total number of cells in duplicate wells every day for 4 to 6 days.

BG01(1) Growth

Growth curve for BG01(1)
Days (in culture) Cells/Well Average #
of Cells		 Standard
Deviation
Day 1 115000
115000
105000
105000		 110000 5774
Day 2 117500
155000
152500
132500		 139375 17722
Day 3 190000
237500
145000
175000		 186875 38588
Day 4 380000
227500
287500
320000		 303750 63656
Day 5 642500
532500
547500
690000		 603125 75674
Day 6 675000
710000
885000
770000		 760000 92105

UC06 Growth

Growth curve for UC06
Days (in culture) Cells/Well Average #
of Cells		 Standard
Deviation		 Percentage
(compared to Day 1)
Experiment 1
Day 1 157500 157500 100.00
Day 2 no data no data no data no data
Day 3 350000 350000 222.22
Day 4 640000 640000 406.35
Day 5 1290000 1290000 819.05
Experiment 2
Day 1 226000
196000		 211000 21213.2 100.00
Day 2 334000
296000		 315000 26870.1 149.29
Day 3 557500
517500		 537500 28284.3 254.74
Day 4 997500
910000		 953750 61871.8 452.01
Experiment 3
Day 1 147500
162500		 155000 10606.6 100.00
Day 2 192000
208000		 200000 11313.7 129.03
Day 3 337500
350000		 343750 8838.8 221.77
Day 4 765000
627500		 696250 97227.2 449.19
Day Average Percentage Standard Deviation
Day 1 100.00 0.00
Day 2 139.16 14.33
Day 3 232.91 18.91
Day 4 435.85 25.59

WA01 Growth

Growth curve for WA01
Days (in culture) Cells/Well Average #
of Cells		 Standard
Deviation
Day 1 1475000
1500000
1025000
1300000		 1325000 218898.7589
Day 2 1425000
1950000
1575000
1700000		 1662500 222204.8604
Day 3 2700000
2575000
3325000
3150000		 2937500 357363.0274
Day 4 3350000
3175000
2575000
3050000		 3037500 331976.4048
Day 5 7800000
9400000
4950000
5400000		 6887500 2090603.98
Day 6 11450000
12250000
8650000
8350000		 10175000 1965324.401

WA07 Growth

Growth curve for WA07
Days (in culture) Cells/Well Average #
of Cells		 Percentage
(compared to Day 1)
Experiment 1
Day 1 77500
105000
95000
97500		 93750 100
Day 2 70000
70000
87500
75000		 75625 80.67
Day 3 165000
180000
185000
152500		 170625 182
Day 4 185000
182500
210000
240000		 204375 218
Day 5 417500
487500
372500
455000		 433125 462
Day 6 No data No data No data
Experiment 2
Day 1 925000
750000
600000
925000
 800000 100
Day 2 1250000
1050000
475000
1125000
 975000 121.88
Day 3 1600000
1675000
1800000
2175000
 1812500 226.56
Day 4 4800000
4650000
4450000
3325000
 4306250 538.28
Day 5 6350000
6450000
6650000
6350000
 6450000 806.25
Day 6 7300000
8150000
7300000
7350000		 7525000 940.63
Day Average Percentage Standard Deviation
Day 1 100 0
Day 2 101.27 29.14
Day 3 204.28 31.51
Day 4 378.14 226.47
Day 5 806.25 243.42
Day 6 940.63 0

WA09 Growth

Growth curve for WA09
Days (in culture) Cells/Well Average #
of Cells		 Standard
Deviation		 Percentage
(compared to Day 1)
Experiment 1
Day 1 146000
156000		 151000 7071.1 100.00
Day 2 188000
212000		 200000 16970.6 132.45
Day 3 495000
585000		 540000 63639.6 357.62
Day 4 745000
532500		 638750 150260.2 423.01
Experiment 2
Day 1 128000
128000		 128000 0 100.00
Day 2 155000
182500		 168750 40750 131.84
Day 3 174000
206000		 190000 21250 148.44
Day 4 520000
537500		 528750 338750 413.09
Day Average Percentage Standard Deviation
Day 1 100.00 0.00
Day 2 132.14 0.43
Day 3 253.03 147.91
Day 4 418.05 7.02