Protocol Number: 93-I-0063

Title:

Study of the Immunopathogenesis, Natural History, and Genetics of Autoimmune Lymphoproliferative Syndrome (ALPS) Associated with an Expansion of CD4-8-/TCR alpha/beta+ T Cells

Number:

93-I-0063

Summary:

The purpose of the protocol is to allow for patients, and relatives of patients, who may have the newly described autoimmune lymphoproliferative syndrome, to be evaluated at the NIH Clinical Center. This evaluation will include blood and relevant tissue studies along with long-term clinical evaluations to define the biology, inheritance,clinical spectrum, and natural history of this syndrome. The aim of the research is to understand mechanisms involved in the development of expanded numbers of what is typically a rare population of immune cells (CD4-8-/TCRalpha/beta+ T cells, otherwise referred to as double negative T cells), and how these relate to the development of expanded numbers of immune cells and autoimmune (self against self) responses in patients with ALPS.

In some cases, we may proivide treatment related to ALPS. These treatments are consistent with standard medical practice.

Participants with ALPS will be invited to visit the NIH once a year or more frequently when clinically indicated for the next few years for clinicians and scientists to follow the course of their disease and to manage its complications. Knowledge gained from these studies provides important insights into the mechanisms of autoimmunity, the thymus gland, and the role that the immune system and genetics plays in ALPS.

Autoimmune lymphoproliferative syndrome is a rare disease that affects both children and adults. Each of these three words helps describe the main features of this condition. The word autoimmune (self-immune) identifies ALPS as a disease of the immune system. The tools used to fight germs turn against our own cells and cause problems. The word lymphoproliferative describes the unusually large numbers of white blood cells (called lymphocytes (stored in the lymph nodes and spleens of people with ALPS. The word syndrome refers to the many common symptoms shared by ALPS patients.

One of the causes of ALPS is defective apoptosis, or said another way, an individual has an abnormality in how well lymphocytes (immune cells) die when they are instructed to do so. It is normal for lymphocytes to disintegrate (e.g., die) when they have done their job. In people with ALPS and in some of their affected relatives, the genetic message for the cells to die is altered: the message is not received and the cells do not die when they should. As a result, people with ALPS develop an enlarged spleen, liver and lymph glands, along with a range of other problems involving white blood cell counts and overactive immune responses (autoimmune disease). Some patients have an increased risk of developing lymphatic cancers (lymphoma).

Provided is a description of eligible study candidates:

1.) Any patient with ALPS, male or female and of any age. As a patient with ALPS, candidates must have:

- a medical history of an enlarged spleen and/or enlarged lymph nodes over an extended period of time (past and/or current).

- defective lymphocyte apoptosis, in vitro.

- greater than or equal to 1 percent TCR alpha/beta+CD4-8- peripheral blood T cells.

2.) Relatives (any age) of patients and normal controls (18-65).

3.) Healthy normal volunteers will also be enrolled to provide data on normal cell behavior for comparison with patients.

Additional information regarding ALPS and the research being conducted at the National Institutes of Health is available at the following World Wide Web (e.g., Internet) locations:

http://www.niaid.nih.gov/publications/alps/

http://www.nhgri.nih.gov/DIR/GMBB/ALPS/.

Sponsoring Institute:

National Institute of Allergy and Infectious Diseases (NIAID)

Recruitment Detail

Type: Participants currently recruited/enrolled

Gender: Male & Female

Referral Letter Required: Yes

Population Exclusion(s): None

Eligibility Criteria:

INCLUSION CRITERIA:

A. ALPS Patient Sample

Study size: up to 800 patients (300 ALPS patients, 500 Relatives and normal controls)

Sex Distribution: Male and female

Age range: All ages acceptable

B. To be considered as having ALPS, patients must have greater than or equal to 1 percent TCR alpha/beta+ CD4-8- peripheral blood T cells, and any one of the following:

1. A history of chronic splenomegaly and/or lymphadenopathy;

2. History of autoimmune cytopenias

3. Patients in a family with multiple individual with symptoms suggestive of ALPS (e.g. high penetrance)

4. Patients with unique clinical features, in our experience that may help us to understand the phenotype.

5. Willingness to allow blood, tissue and other samples to be stored.

C. Relatives (any age) of patients and normal controls.

To determine if there is a genetic basis for this syndrome, we may obtain blood for research studies on unaffected relative of these patients. We may also perform some or all of the studies listed in section IV of the protocol on blood samples from normal relatives. We may also obtain tissue specimens from unrelated individuals who do not have this lymphoproliferative syndrome but have undergone medically indicated diagnostic or therapeutic resection of lymphoid tissue. Lymphoid tissue from unrelated individuals will be collected in such a way that the subject cannot be identified directly or indirectly. Lymphoid tissue may include some or all of those listed in section V of the protocol. Up to 500 male and female relatives of ALPS patients or control subjects will be studied.

D. Recruitment Strategies: Patients and families are referred from NIH investigators, immunologists, hematologists, general medical doctors and geneticists who learn of our studies through our scientific presentations and publications; family organizations, such as the Immune Deficiency Foundation; and the internet.

EXCLUSION CRITERIA:

1. HIV infection in any potential subject, and recognized or suspected immunologic disorders or complicating medical or psychiatric conditions in potential unrelated controls.

2. Any condition that the Principal Investigator deems to be non-conducive to the research goals of the study.

Special Instructions:

This protocol studies the long term effects of your disease. Therefore, you will be invited to visit the NIH once a year or more frequently for the next few years so that we can follow the progression of your disease and manage its complications.

Keywords:

Immunoregulation

T Cell Receptor Alpha/Beta

Lymphadenopathy

Recruitment Keyword(s):

Autoimmune Lymphoproliferative Syndrome

ALPS

Condition(s):

Autoimmune Disease

Lymphatic Disease

Lymphoproliferative Disorder

Canale-Smith Syndrome

Investigational Drug(s):

None

Investigational Device(s):

None

Intervention(s):

None

Supporting Site:

National Institute of Allergy and Infectious Diseases

Contact(s):

Patient Recruitment and Public Liaison Office
Building 61
10 Cloister Court
Bethesda, Maryland 20892-4754
Toll Free: 1-800-411-1222
TTY: 301-594-9774 (local),1-866-411-1010 (toll free)
Fax: 301-480-9793

Electronic Mail:prpl@mail.cc.nih.gov

Citation(s):

A novel lymphoproliferative/autoimmune syndrome resembling murine lpr/gld disease

Dominant interfering gas gene mutations impair apoptosis in a human autoimmune lymphoproliferative syndrome

Mutations in Fas associated with human lymphoproliferative syndrome and autoimmunity

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