With the success of the first two incarnations of LoBoS, there was an increase in interest, both at NIH and other institutions, in using Beowulf clusters to conduct biochemical research. To meet the challenges posed by this new avenue of research and the increasing demand for cluster resources, CIT and NHLBI at NIH decided to combine forces to design a new cluster. The Cluster 2000 committee was therefore chartered to conduct the design work. The result of this collaboration was a combined LoBoS 3 and Biowulf cluster.

Biowulf itself is maintained by the same organization as the NIH Helix systems.

LoBoS nodes installed as part of Biowulf.