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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR1175 IGR1173 IGR1172 IGR1174 IGR1176 IGR1177 IGR1179 IGR1178 lacF,celC, - SMu1357 lacA, - SMu1361 lacB,rpiB, - SMu1360 lacR, - SMu1362 lacC, - SMu1359 lacD, - SMu1358 lacG, - SMu1355 lacE, - SMu1356 rexA, - SMu1363 lacF,celC, - SMu1357 lacA, - SMu1361 lacB,rpiB, - SMu1360 lacR, - SMu1362 lacC, - SMu1359 lacD, - SMu1358 lacG, - SMu1355 lacE, - SMu1356 rexA, - SMu1363 Type: tandem, Name: SMu-TR-24 - 24 Type: tandem, Name: SMu-TR-24 - 24 lacF,celC, - SMu1357 lacA, - SMu1361 lacB,rpiB, - SMu1360 lacR, - SMu1362 lacC, - SMu1359 lacD, - SMu1358 lacG, - SMu1355 lacE, - SMu1356 rexA, - SMu1363


LANL Gene ID: SMu1360

GenBank Locus Tag: SMU.1495c

DNA Molecule Name:
1  

GenBank ID:


Gene Name:
lacB  rpiB  

Definition:
galactose-6-phosphate isomerase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
1420157

Gene Stop:
1419642

Gene Length:
516

Molecular Weight*:
18934

pI*:
6.20

Net Charge*:
-3.46

EC:
5.3.1.26  

Functional Class:
Energy metabolism; Sugars  

Gene Ontology:
Biological process
  GO:0005975    carbohydrate metabolic process
  GO:0005990    lactose catabolic process

Molecular function
  GO:0016861    intramolecular oxidoreductase activity, interconverting aldoses and ketoses


Pathway: pathway table
Galactose metabolism

Primary Evidence:
Rosey,E.L. and Stewart,G.C.
Nucleotide and deduced amino acid sequences of the lacR, lacABCD,
and lacFE genes encoding the repressor, tagatose 6-phosphate gene
cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans
J. Bacteriol. 174 (19), 6159-6170 (1992)
PubMed: 1400164


Secondary Evidence:
Rosey,E.L., Oskouian,B. and Stewart,G.C.
Lactose metabolism by Staphylococcus aureus: characterization of
lacABCD, the structural genes of the tagatose 6-phosphate pathway
J. Bacteriol. 173 (19), 5992-5998 (1991)
PubMed: 1655695

van Rooijen,R.J., van Schalkwijk,S. and de Vos,W.M.
Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis
J. Biol. Chem. 266 (11), 7176-7181 (1991)
PubMed: 1901863



Comment:
For other 'lac' genes see SMu0104 (lacD); SMu0793 (lacR); SMu0794 (lacC); SMu0094 (lacI); SMu1359 (lacC);SMu1354 (lacX); SMu1355 (lacG); SMu1356 (lacE);SMu1358 (lacD);SMu1361 (lacA);SMu1362 (lacR) and SMu1451 (lacE).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
This sequence corresponds to the previously published 322118|, 125920|,153674| and 282311| in GenBank. It is similar to other predicted galactose-6-phosphate isomerase in gapped BLAST, e.g. residues 1-171 are 80% similar to 125919| from S.aureus.

Weaker similarity to rpiB of E.coli.




The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1930 (1e-69).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379888|ref|NP_721843.1|  galactose-6-phosphate isomeras...   346   4e-94
 gi|153674|gb|AAA26905.1|  galactose 6-P isomerase                 343   3e-93
 gi|27468704|ref|NP_765341.1|  galactose-6-phosphate isomeras...   308   6e-83
 gi|70725844|ref|YP_252758.1|  galactose-6-phosphate isomeras...   308   7e-83
 gi|82751792|ref|YP_417533.1|  galactose-6-phosphate isomeras...   292   4e-78
 gi|21283849|ref|NP_646937.1|  galactose-6-phosphate isomeras...   292   6e-78
 gi|15925184|ref|NP_372718.1|  galactose-6-phosphate isomeras...   287   1e-76
 gi|157074495|gb|ABV09178.1|  galactose-6-phosphate isomerase...   278   1e-73
 gi|15903118|ref|NP_358668.1|  galactose-6-phosphate isomeras...   277   2e-73
 gi|15901057|ref|NP_345661.1|  galactose-6-phosphate isomeras...   276   2e-73


InterPro Summary:  InterProScan

InterPro
IPR003500
Family
Ribose/galactose isomerase
PIRSF005384 [1-149]T 8.3000000000005E-77 PIRSF005384 RpiB_LacA_B RpiB_LacA_B
PF02502 [2-142]T 1.0E-61 PF02502 LacAB_rpiB LacAB_rpiB
TIGR00689 [3-147]T 2.2997204332343502E-34 TIGR00689 rpiB_lacA_lacB rpiB_lacA_lacB
InterPro
IPR004784
Family
Galactose-6-phosphate isomerase LacB subunit
TIGR01119 [1-171]T 0.0 TIGR01119 lacB lacB
noIPR
unintegrated
unintegrated
G3DSA:3.40.1400.10 [1-158]T 1.4999964819631799E-49 G3DSA:3.40.1400.10 G3DSA:3.40.1400.10 G3DSA:3.40.1400.10
SSF89623 [1-155]T 2.2E-52 SSF89623 SSF89623 SSF89623


COGS Summary:  COGS Search
BeTs to 9 clades of COG0698
COG name: Ribose 5-phosphate isomerase RpiB
Functional Class: G
The phylogenetic pattern of COG0698 is -----qvcebr-ujgp----x
Number of proteins in this genome belonging to this COG is 3
Blocks Summary:  Blocks Search
***** IPB003500 (Ribose/Galactose Isomerase) with a combined E-value of 2.6e-45.
    IPB003500A    1-31
    IPB003500B    57-98


 ProDom Summary:  Protein Domain Search
Residues 143-171 are identical to a (ISOMERASE SUBUNIT METABOLISM LACTOSE) protein domain (PD020209 which is seen in LACB_STRMU.

Residues 55-142 are 79% similar to a (ISOMERASE COMPLETE PROTEOME RIBOSE) protein domain (PD004509 which is seen in LACB_LACLA.

Residues 61-107 are 48% similar to a (ISOMERASE PROTEOME COMPLETE) protein domain (PD223670 which is seen in Q9PRD9_UREPA.

Residues 1-128 are 31% similar to a (ISOMERASE SUBUNIT METABOLISM LACTOSE) protein domain (PD020210 which is seen in LACA_LACLA.

Residues 2-52 are 96% similar to a (ISOMERASE PROTEOME COMPLETE METABOLISM) protein domain (PD010571 which is seen in LACB_STRMU.


Paralogs:  Local Blast Search
SMu1360 is paralogously related (blast p-value < 1e-3) to SMu1361, a predicted galactoside O-acetyltransferase.


Pfam Summary:  Pfam Search
Residues 61 to 142 (E-value = 6.1e-48) place SMu1360 in the LacAB_rpiB family which is described as Ribose/Galactose Isomerase (PF02502)

 Structural Feature(s):
Feature Type  Start  Stop
transmembrane  
103  
119

Top PDB Hits:
No significant hits to the NCBI PDB database.

Gene Protein Sequence:
MKIAIGCDHIVTDVKMELSKHLKEEGYEVLDVGTYDFTRTHYPIFGKKVG
EAVSSGEADLGVCMCGTGVGISNAANKVPGVRTALVRDMTSALYSKEELN
ANVVSFGGAIIGKLLLFDIVDAFIKAQYKPTEENKKLIAKIKHLEAHNDK
QADPHFFDEFLEKWNRGDYHD

Gene Nucleotide Sequence:  Sequence Viewer
ATGAAAATTGCTATTGGTTGTGACCATATTGTTACTGATGTGAAAATGGA
ACTATCTAAACATCTAAAAGAAGAAGGTTATGAAGTTCTAGATGTAGGGA
CCTATGACTTTACACGTACGCACTATCCCATCTTTGGTAAAAAAGTGGGC
GAAGCAGTTAGCAGTGGTGAAGCGGATTTAGGTGTATGTATGTGCGGTAC
TGGTGTGGGTATAAGTAATGCTGCCAATAAAGTACCGGGTGTTAGAACGG
CATTAGTAAGAGATATGACTTCTGCACTGTATTCAAAAGAAGAACTTAAT
GCAAATGTCGTTAGCTTTGGCGGCGCTATTATAGGTAAGTTGTTATTATT
TGATATTGTAGATGCTTTTATTAAAGCCCAGTATAAACCGACAGAAGAAA
ATAAGAAATTAATTGCTAAGATCAAACATTTAGAAGCACATAACGATAAA
CAAGCAGATCCGCATTTCTTTGATGAATTTCTAGAAAAGTGGAATCGCGG
TGACTATCACGATTGA

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