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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR474 IGR475 IGR476 IGR473 IGR477 IGR479 IGR478 yddK, - SMu0550 SMu0551 SMu0553 SMu0552 murF, - SMu0549 prfC, - SMu0554 bsp, - SMu0555 spaP,sr,pags-5,pac, - SMu0556 yddK, - SMu0550 SMu0551 SMu0553 SMu0552 murF, - SMu0549 prfC, - SMu0554 bsp, - SMu0555 spaP,sr,pags-5,pac, - SMu0556 SMu0551 SMu0553 SMu0552 murF, - SMu0549 prfC, - SMu0554 bsp, - SMu0555 spaP,sr,pags-5,pac, - SMu0556 yddK, - SMu0550


LANL Gene ID: SMu0554

GenBank Locus Tag: SMU.608

DNA Molecule Name:
1  

GenBank ID:
24379085

Gene Name:
prfC  

Definition:
peptide chain release factor 3 ; translation elongation and release factor

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
565446

Gene Stop:
566990

Gene Length:
1545

Molecular Weight*:
58555

pI*:
4.90

Net Charge*:
-17.76

EC:
 

Functional Class:
Protein synthesis; Translation factors  

Gene Ontology:
Biological process
  GO:0006412    translation
  GO:0006415    translational termination

Cellular component
  GO:0005622    intracellular
  GO:0005737    cytoplasm

Molecular function
  GO:0003924    GTPase activity
  GO:0005525    GTP binding
  GO:0016149    translation release factor activity, codon specific


Pathway: pathway table

Primary Evidence:
Ogier,J.A., Scholler,M., Lepoivre,Y., Gangloff,S., M'Zoughi,R. and
Klein,J.P.
A 40-kilodalton cell wall protein-coding sequence upstream of the
sr gene of Streptococcus mutans OMZ175 (serotype f)
Infect. Immun. 59 (5), 1620-1626 (1991)
PubMed: 2019433

LeBlanc,D.J., Lee,L.N., Titmas,B.M., Smith,C.J. and Tenover,F.C.
Nucleotide sequence analysis of tetracycline resistance gene tetO
from Streptococcus mutans DL5
J. Bacteriol. 170 (8), 3618-3626 (1988)
PubMed: 2841293


Secondary Evidence:
Kawazu,Y., Ito,K., Matsumura,K. and Nakamura,Y.
Comparative characterization of release factor RF-3 genes of
Escherichia coli, Salmonella typhimurium, and Dichelobacter nodosus
J. Bacteriol. 177 (19), 5547-5553 (1995)
PubMed: 7559341

Mikuni,O., Ito,K., Moffat,J., Matsumura,K., McCaughan,K.,
Nobukuni,T., Tate,W. and Nakamura,Y.
Identification of the prfC gene, which encodes
peptide-chain-release factor 3 of Escherichia coli
Proc. Natl. Acad. Sci. U.S.A. 91 (13), 5798-5802 (1994)
PubMed: 8016068

Grentzmann,G., Brechemier-Baey,D., Heurgue,V., Mora,L. and
Buckingham,R.H.
Localization and characterization of the gene encoding release
factor RF3 in Escherichia coli
Proc. Natl. Acad. Sci. U.S.A. 91 (13), 5848-5852 (1994)
PubMed: 8016077

Mortensen,K.K., Hansen,H.F., Grentzmann,G., Buckingham,R.H. and
Sperling-Petersen,H.U.
Osmo-expression and fast two-step purification of Escherichia coli
translation termination factor RF-3
Eur. J. Biochem. 234 (3), 732-736 (1995)
PubMed: 8575429

Grentzmann,G., Brechemier-Baey,D., Heurgue-Hamard,V. and
Buckingham,R.H.
Function of polypeptide chain release factor RF-3 in Escherichia
coli. RF-3 action in termination is predominantly at UGA-containing stop signals
J. Biol. Chem. 270 (18), 10595-10600 (1995)
PubMed: 7737996





Comment:
For other 'prf' genes see SMu0993 (prfA) and SMu1211 (prfB).

From Genbank:[gi:13878664]
This protein increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2.It binds guanine nucleotides and has strong preference for UGA stop codons.It may interact directly with the ribosome.The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP,but not by GMP.



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Matches in gapped BLAST to translation elongation factor EF-G homolog:residues 1-514 are 99% similar to the previously published protein in S.mutans (gi280249).

Residues 1-514 are 87% similar to peptide chain release factor 3 in S.pneumoniae (gi15900357) and are 86% similar to the protein from S.pyogenes (gi15675335).






The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0772 (0.0).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379085|ref|NP_721040.1|  peptide chain release factor 3...   986   0.0  
 gi|27543520|dbj|BAC54562.1|  regulator of gtfB [Streptococcu...   986   0.0  
 gi|157076035|gb|ABV10718.1|  peptide chain release factor 3 ...   954   0.0  
 gi|149023725|ref|ZP_01836205.1|  peptide chain release facto...   951   0.0  
 gi|15900357|ref|NP_344961.1|  peptide chain release factor 3...   950   0.0  
 gi|148988190|ref|ZP_01819653.1|  peptide chain release facto...   950   0.0  
 gi|148983833|ref|ZP_01817152.1|  peptide chain release facto...   949   0.0  
 gi|15902440|ref|NP_357990.1|  peptide chain release factor 3...   949   0.0  
 gi|149012188|ref|ZP_01833297.1|  peptide chain release facto...   949   0.0  
 gi|149003536|ref|ZP_01828410.1|  peptide chain release facto...   948   0.0


InterPro Summary:  InterProScan

InterPro
IPR000795
Domain
Protein synthesis factor, GTP-binding
PR00315 [12-25]T 1.3E-12 PR00315 ELONGATNFCT[62-70]T 1.3E-12 PR00315 ELONGATNFCT[82-92]T 1.3E-12 PR00315 ELONGATNFCT[98-109]T 1.3E-12 PR00315 ELONGATNFCT[134-143]T 1.3E-12 PR00315 ELONGATNFCT ELONGATNFCT
PF00009 [8-268]T 8.200013071350031E-64 PF00009 GTP_EFTU GTP_EFTU
PS00301 [55-70]T 8.0E-5 PS00301 EFACTOR_GTP EFACTOR_GTP
InterPro
IPR002127
Family
Tetracycline-resistance protein
PR01037 [120-143]T 3.1E-10 PR01037 TCRTETOQM[239-261]T 3.1E-10 PR01037 TCRTETOQM TCRTETOQM
InterPro
IPR004161
Domain
Translation elongation factor EFTu/EF1A, domain 2
PF03144 [302-369]T 1.59999889349251E-10 PF03144 GTP_EFTU_D2 GTP_EFTU_D2
InterPro
IPR004548
Family
Peptide chain release factor 3
TIGR00503 [2-513]T 0.0 TIGR00503 prfC prfC
InterPro
IPR005225
Domain
Small GTP-binding protein domain
TIGR00231 [8-185]T 1.1897066421818402E-18 TIGR00231 small_GTP small_GTP
InterPro
IPR009000
Domain
Translation elongation and initiation factors/Ribosomal, beta-barrel
SSF50447 [250-379]T 5.400000000000001E-28 SSF50447 Translat_factor Translat_factor
InterPro
IPR009022
Domain
Elongation factor G, III and V
SSF54980 [380-455]T 1.4E-17 SSF54980 EFG_III_V EFG_III_V
noIPR
unintegrated
unintegrated
G3DSA:2.40.30.10 [282-379]T 2.3000044072653903E-22 G3DSA:2.40.30.10 G3DSA:2.40.30.10 G3DSA:2.40.30.10
G3DSA:3.30.70.870 [381-458]T 1.3999996890690802E-4 G3DSA:3.30.70.870 G3DSA:3.30.70.870 G3DSA:3.30.70.870
G3DSA:3.40.50.300 [2-270]T 5.2999732988553305E-74 G3DSA:3.40.50.300 G3DSA:3.40.50.300 G3DSA:3.40.50.300
PTHR23115 [1-161]T 4.600000443306959E-112 PTHR23115 PTHR23115[226-402]T 4.600000443306959E-112 PTHR23115 PTHR23115 PTHR23115
PTHR23115:SF13 [1-161]T 4.600000443306959E-112 PTHR23115:SF13 PTHR23115:SF13[226-402]T 4.600000443306959E-112 PTHR23115:SF13 PTHR23115:SF13 PTHR23115:SF13
SSF52540 [1-274]T 1.8E-72 SSF52540 SSF52540 SSF52540


COGS Summary:  COGS Search
BeTs to 17 clades of COG0480
COG name: Translation elongation and release factors (GTPases)
Functional Class: J
The phylogenetic pattern of COG0480 is amtkYqVCEbRHujgpOLinx
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
***** IPB000640 (Elongation factor G, C-terminus) with a combined E-value of 1e-37.
    IPB000640A    10-35
    IPB000640B    54-76
    IPB000640C    81-108
    IPB000640D    115-143
***** IPB000795 (GTP-binding elongation factor) with a combined E-value of 2.2e-19.
    IPB000795A    12-27
    IPB000795B    84-115
    IPB000795C    119-143
***** IPB000178 (Initiation factor 2) with a combined E-value of 1.1e-12.
    IPB000178A    9-47
    IPB000178B    81-113
    IPB000178C    114-153


 ProDom Summary:  Protein Domain Search
Residues 114-191 are 41% similar to a (GTP-BINDING FACTOR INITIATION COMPLETE PROTEOME) protein domain (PD200055 which is seen in RF3_BUCAI.

Residues 93-181 are 62% similar to a (FACTOR RELEASE GTP-BINDING CHAIN) protein domain (PD013442 which is seen in RF3_LACLA.

Residues 413-510 are 77% similar to a (RELEASE PEPTIDE FACTOR CHAIN) protein domain (PD013443 which is seen in RF3_LACLA.

Residues 234-368 are 83% similar to a (RESISTANCE GTP-BINDING BIOSYNTHESIS ANTIBIOTIC) protein domain (PD002777 which is seen in RF3_LACLA.

Residues 60-92 are 96% similar to a (FACTOR GTP-BINDING BIOSYNTHESIS) protein domain (PD336744 which is seen in RF3_LACLA.

Residues 1-58 are 91% similar to a (FACTOR ELONGATION GTP-BINDING) protein domain (PD334795 which is seen in RF3_LACLA.

Residues 413-510 are 56% similar to a (PEPTIDE GTP-BINDING FACTOR CHAIN) protein domain (PD396095 which is seen in RF3_STAAU.

Residues 11-92 are 34% similar to a (G GTP-BINDING FACTOR ELONGATION) protein domain (PD249398 which is seen in EFGL_THEMA.

Residues 11-46 are 72% similar to a (GTP-BINDING FACTOR ELONGATION BIOSYNTHESIS RESISTANCE) protein domain (PD000122 which is seen in RF3_SYNY3.

Residues 371-412 are 83% similar to a (GTP-BINDING FACTOR CHAIN RELEASE) protein domain (PD410050 which is seen in RF3_LACLA.


Paralogs:  Local Blast Search
SMu0554 is paralogously related (blast p-value < 1e-3) to SMu0324,a predicted translation elongation factor G, SMu1271 and SMu0496, both predicted GTP-binding proteins, SMu0382,a predicted translation initiation factor, SMu0651,a predicted translation elongation factor EF-Tu, and SMu1471,a predicted GTP-binding protein Era homolog.


Pfam Summary:  Pfam Search
Residues 8 to 268 (E-value = 2.9e-67) place SMu0554 in the GTP_EFTU family which is described as Elongation factor Tu GTP binding domain (PF00009)
Residues 302 to 369 (E-value = 6.6e-13) place SMu0554 in the GTP_EFTU_D2 family which is described as Elongation factor Tu domain 2 (PF03144)

 Top PDB Hits:
pdb1FNMA Chain A, Structure Of Thermus Thermophilus Ef-G H573a 182 9e-047
pdb1ELO Elongation Factor G Without Nucleotide >gi1633250... 182 9e-047
pdb2EFGA Chain A, Translational Elongation Factor G Complexed... 180 4e-046

Gene Protein Sequence:
MNLQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKTGN
FAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTL
MAVDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPIFTFINKLDRDGREPLD
LLEELEEVLGIASYPMNWPIGMGRSFEGLYDLYNQRLELYKGDERFASIE
DGDKLFANNPFYEQAKEDIELLTEAGNEFSEEAILKGELTPVFFGSALTN
FGVQTFLDSFLAFAPEPHGHKTTDDKVIDPLDKDFSGFVFKIQANMDPRH
RDRIAFVRIVSGEFERGMSVNLARTGKSVKLSNVTQFMAESRENVENAVA
GDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVSAKNVMKQK
SFHKGIEQLVQEGAIQLYTNYQTGEYMLGAVGQLQFEVFKHRMENEYNAE
VIMTPMGKKTVRWIKEEDLDERMSSSRNILAKDRFNKPVFLFENDFSLHW
FADKYPDIVLEEKM

Gene Nucleotide Sequence:  Sequence Viewer
ATGAACTTACAAGAAGAAATTAAGAAACGTCGAACCTTTGCCATCATTTC
CCACCCGGATGCTGGTAAGACGACGATTACGGAGCAGTTGCTCTATTTTG
GAGGTGAAATCCGAGAGGCTGGTACTGTTAAAGGAAAGAAAACCGGTAAT
TTTGCCAAATCTGACTGGATGGATATTGAAAAACAGCGTGGGATTTCCGT
AACTTCATCTGTCATGCAGTTTGACTATGCGGGTAAGCGGGTCAATATCC
TTGATACACCTGGGCATGAGGACTTTTCTGAGGATACTTACCGAACACTA
ATGGCGGTAGATGCTGCTGTTATGGTCGTGGACTCTGCCAAGGGGATCGA
AGCACAGACAAAAAAGCTTTTTGAAGTTGTCAAGCATCGTGGTATCCCTA
TCTTTACTTTTATCAATAAATTGGACCGTGATGGTCGCGAACCGCTGGAT
TTATTGGAAGAATTAGAAGAGGTATTGGGGATTGCTTCTTATCCTATGAA
TTGGCCTATTGGTATGGGAAGATCTTTTGAGGGGCTCTATGATTTGTACA
ATCAGCGTCTTGAACTCTACAAAGGTGATGAGCGTTTTGCTTCTATTGAA
GATGGTGACAAGCTCTTTGCTAATAATCCCTTCTATGAACAAGCTAAAGA
AGATATTGAACTTTTGACTGAAGCGGGAAATGAATTTTCAGAAGAAGCCA
TTTTAAAGGGTGAGCTGACGCCTGTCTTCTTTGGCTCAGCCCTAACCAAC
TTTGGTGTTCAAACTTTCTTGGATTCTTTCTTAGCATTTGCACCAGAACC
ACACGGTCACAAGACCACTGACGATAAGGTGATTGATCCTTTAGATAAAG
ACTTTTCAGGCTTTGTTTTTAAAATCCAAGCTAATATGGATCCTCGCCAC
CGAGATCGTATTGCCTTTGTGCGGATTGTCTCAGGTGAATTTGAACGCGG
CATGTCTGTCAACCTTGCCCGCACTGGTAAGAGCGTTAAGCTGTCAAACG
TCACTCAGTTTATGGCAGAATCTCGTGAGAATGTAGAAAATGCTGTCGCT
GGTGATATTATCGGAGTTTACGATACAGGAACTTATCAGGTTGGTGATAC
CCTAACTGTTGGTAAAAATAAATTTGAATTTGAGCCACTGCCGACCTTTA
CACCAGAGCTCTTTATGAAAGTTTCTGCTAAAAATGTTATGAAGCAGAAG
TCTTTTCATAAAGGCATTGAGCAATTGGTGCAAGAAGGTGCTATACAGCT
TTATACCAACTATCAAACTGGTGAATATATGCTTGGAGCAGTCGGTCAGC
TCCAGTTTGAAGTTTTCAAACACCGCATGGAAAATGAATACAATGCGGAG
GTTATCATGACACCCATGGGTAAGAAAACGGTGCGTTGGATTAAGGAGGA
AGATCTTGATGAACGTATGTCTTCCAGCCGCAATATATTGGCTAAAGACC
GCTTTAACAAGCCCGTTTTCCTCTTTGAAAATGACTTTTCTCTTCATTGG
TTTGCAGATAAATATCCAGATATAGTTTTGGAAGAGAAGATGTAA

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