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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR370 IGR372 IGR373 IGR375 IGR376 IGR368 IGR369 IGR374 pbp1a,pbpA, - SMu0424 SMu0414 SMu0419 SMu0418 SMu0417 SMu0416 trxB, - SMu0420 pepC, - SMu0423 rheB,yqfR, - SMu0415 yueK,pncB, - SMu0421 pbp1a,pbpA, - SMu0424 SMu0414 SMu0419 SMu0418 SMu0417 SMu0416 trxB, - SMu0420 pepC, - SMu0423 rheB,yqfR, - SMu0415 yueK,pncB, - SMu0421 Type: tandem, Name: SMu-TR-8 - 8 pbp1a,pbpA, - SMu0424 SMu0414 SMu0418 SMu0417 SMu0416 trxB, - SMu0420 pepC, - SMu0423 rheB,yqfR, - SMu0415 yueK,pncB, - SMu0421 nadE, - SMu0422 nadE, - SMu0422 SMu0419


LANL Gene ID: SMu0420

GenBank Locus Tag: SMU.463

DNA Molecule Name:
1  

GenBank ID:
24378951

Gene Name:
trxB  

Definition:
thioredoxin reductase (NADPH)

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
430700

Gene Stop:
431614

Gene Length:
915

Molecular Weight*:
33104

pI*:
4.70

Net Charge*:
-11.87

EC:
1.8.1.9  

Functional Class:
Energy metabolism; Electron transport  

Gene Ontology:
Biological process
  GO:0006118    electron transport
  GO:0019430    removal of superoxide radicals

Cellular component
  GO:0005737    cytoplasm

Molecular function
  GO:0004791    thioredoxin-disulfide reductase activity
  GO:0016491    oxidoreductase activity
  GO:0050660    FAD binding


Pathway: pathway table
Pyrimidine metabolism

Secondary Evidence:
Powis G, Montfort WR.
Properties and biological activities of thioredoxins.
Annu Rev Biophys Biomol Struct. 2001;30:421-55. Review.
PMID: 11441809

Comment:
For other 'trx' genes see SMu0792 (trxB); SMu1789 (trxH) and SMu1699 (trxA).

View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Matches in gapped BLAST to thioredoxin reductases. Residues 1-303 are 78% similar to gi15903355 from S.pyogenes and 77% similar to gi15901308 from S.pneumoniae.





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG0294 (1e-133).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24378951|ref|NP_720906.1|  putative thioredoxin reductase...   598   e-169
 gi|21910931|ref|NP_665199.1|  putative thioredoxin reductase...   489   e-137
 gi|94990986|ref|YP_599086.1|  Thioredoxin reductase [Strepto...   488   e-136
 gi|71904063|ref|YP_280866.1|  thioredoxin reductase [Strepto...   488   e-136
 gi|19746575|ref|NP_607711.1|  thioredoxin reductase [Strepto...   487   e-136
 gi|71911173|ref|YP_282723.1|  thioredoxin reductase [Strepto...   486   e-136
 gi|50914752|ref|YP_060724.1|  Thioredoxin reductase [Strepto...   486   e-136
 gi|139473303|ref|YP_001128018.1|  putative thioredoxin reduc...   485   e-135
 gi|15903355|ref|NP_358905.1|  Thioredoxin reductase [Strepto...   473   e-132
 gi|15901308|ref|NP_345912.1|  thioredoxin reductase [Strepto...   473   e-132


InterPro Summary:  InterProScan

InterPro
IPR000103
Domain
Pyridine nucleotide-disulphide oxidoreductase, class-II
PR00469 [3-25]T 2.9E-72 PR00469 PNDRDTASEII[36-51]T 2.9E-72 PR00469 PNDRDTASEII[56-66]T 2.9E-72 PR00469 PNDRDTASEII[101-109]T 2.9E-72 PR00469 PNDRDTASEII[123-135]T 2.9E-72 PR00469 PNDRDTASEII[138-162]T 2.9E-72 PR00469 PNDRDTASEII[190-206]T 2.9E-72 PR00469 PNDRDTASEII[229-250]T 2.9E-72 PR00469 PNDRDTASEII[263-281]T 2.9E-72 PR00469 PNDRDTASEII PNDRDTASEII
InterPro
IPR000759
Family
Adrenodoxin reductase
PR00419 [3-25]T 1.1E-6 PR00419 ADXRDTASE[143-157]T 1.1E-6 PR00419 ADXRDTASE ADXRDTASE
InterPro
IPR001327
Domain
Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region
PD000139 [107-175]T 0.0 PD000139 FAD_pyr_redox FAD_pyr_redox
PF00070 [142-235]T 5.799996538547799E-24 PF00070 Pyr_redox Pyr_redox
InterPro
IPR005982
Domain
Thioredoxin reductase
TIGR01292 [2-300]T 0.0 TIGR01292 TRX_reduct TRX_reduct
InterPro
IPR008255
Active_site
Pyridine nucleotide-disulphide oxidoreductase, class-II, active site
PS00573 [130-150]T 8.0E-5 PS00573 PYRIDINE_REDOX_2 PYRIDINE_REDOX_2
InterPro
IPR013027
Domain
FAD-dependent pyridine nucleotide-disulphide oxidoreductase
PR00368 [3-25]T 1.1E-26 PR00368 FADPNR[103-112]T 1.1E-26 PR00368 FADPNR[142-167]T 1.1E-26 PR00368 FADPNR[228-242]T 1.1E-26 PR00368 FADPNR[268-275]T 1.1E-26 PR00368 FADPNR FADPNR
PF07992 [3-278]T 5.20000089003276E-38 PF07992 Pyr_redox_2 Pyr_redox_2
noIPR
unintegrated
unintegrated
G3DSA:3.50.50.60 [1-300]T 7.200027197789859E-55 G3DSA:3.50.50.60 G3DSA:3.50.50.60 G3DSA:3.50.50.60
PTHR22912 [5-298]T 1.70000295590065E-27 PTHR22912 PTHR22912 PTHR22912
SSF51905 [1-300]T 1.9E-58 SSF51905 SSF51905 SSF51905


COGS Summary:  COGS Search
BeTs to 17 clades of COG0492
COG name: Thioredoxin reductase/alkyl hydroperoxide reductase
Functional Class: O
The phylogenetic pattern of COG0492 is amtkYQVcEBrhUJgpolinX
Number of proteins in this genome belonging to this COG is 3
Blocks Summary:  Blocks Search
***** IPB000103 (Pyridine nucleotide-disulphide oxidoreductase class-II) with a combined E-value of 5.1e-69.
    IPB000103A    3-23
    IPB000103B    36-50
    IPB000103C    108-156
    IPB000103D    229-240
    IPB000103E    263-300
    IPB000103A    142-162
***** PR00368 (FAD-dependent pyridine nucleotide reductase signature) with a combined E-value of 5.7e-31.
    PR00368A    3-25
    PR00368B    103-112
    PR00368C    142-167
    PR00368D    228-242
    PR00368E    268-275
***** IPB000171 (Bacterial-type phytoene dehydrogenase) with a combined E-value of 4.2e-07.
    IPB000171A    5-35
    IPB000171A    144-174


 ProDom Summary:  Protein Domain Search
No significant hits to the ProDom database.

Paralogs:  Local Blast Search
SMu0420 is paralogously related (blast p-value < 1e-3) to SMu0693,
a predicted NADH oxidase/alkyl hydroperoxidase reductase, and to SMu0792, a predicted thioredoxin reductase, and to SMu1299, SMu0116, SMu0762, SMu0355, and SMu1020, with various functions.


Pfam Summary:  Pfam Search
Residues 3 to 284 (E-value = 8.1e-74) place SMu0420 in the Pyr_redox family which is described as Pyridine nucleotide-disulphide oxidoreductase (PF00070)

 Top PDB Hits:
pdb1VDC Structure Of Nadph Dependent Thioredoxin Reductase 218 8e-058
pdb1CL0A Chain A, Crystal Structure Of Reduced Thioredoxin Re... 199 4e-052
pdb1TDE Thioredoxin Reductase (E.C.1.6.4.5) (Wild Type) 199 4e-052

Gene Protein Sequence:
MYDTIIIGSGPAGMTAALYAARSNLKVALIEQGAPGGQMNNTSDIENYPG
YDLISGPELSMKMHEPLEKFGVENLYGIVTAVEDHGNFKKVLTDDNSYET
KTVIIATGAKHRPLAVAGEETYNSRGVSYCAVCDGAFFRGQDLLVVGGGD
SAVEEALFLTRFANKVTIVHRRDELRAQKVLQERAFANDKVDFIWDSVVK
EIKGNDLKVTNVDIENVKTGQVNNYAFGGVFIYVGLDPVSSMVKELDITD
EAGWIPTDDHMKTKVAGVFAIGDVRQKDLRQITTAVGDGAVAAQEAYQYI
VNNY

Gene Nucleotide Sequence:  Sequence Viewer
ATGTACGATACAATTATTATTGGCTCAGGTCCTGCTGGCATGACAGCTGC
CCTCTATGCTGCTAGAAGCAATCTCAAAGTTGCTCTAATAGAACAAGGTG
CGCCCGGCGGTCAAATGAATAATACCTCTGATATTGAAAATTATCCCGGT
TATGACTTGATTTCTGGACCAGAACTATCAATGAAAATGCATGAACCACT
TGAAAAATTTGGTGTTGAAAATCTCTATGGTATTGTAACCGCTGTTGAAG
ATCATGGTAATTTCAAGAAGGTCCTTACGGATGACAATAGCTATGAAACC
AAAACTGTTATCATTGCTACAGGTGCTAAACACCGCCCTCTTGCTGTCGC
TGGTGAAGAAACCTATAATAGTCGCGGTGTTTCTTACTGTGCTGTTTGTG
ATGGTGCTTTTTTCCGCGGCCAGGACTTGTTAGTTGTTGGTGGTGGCGAT
TCAGCCGTTGAGGAAGCTCTTTTCTTAACCAGATTTGCCAACAAAGTTAC
TATTGTCCATCGCCGTGATGAACTGCGTGCGCAAAAAGTATTGCAAGAAC
GTGCCTTTGCCAATGATAAAGTAGACTTTATCTGGGATTCTGTTGTTAAA
GAAATCAAAGGGAATGATCTTAAGGTAACCAATGTTGATATTGAAAATGT
CAAAACAGGTCAAGTAAACAATTATGCCTTTGGTGGTGTCTTTATCTATG
TTGGCTTAGATCCTGTCTCAAGTATGGTTAAGGAATTAGATATCACAGAT
GAAGCTGGTTGGATTCCAACAGATGATCATATGAAGACGAAAGTAGCAGG
CGTTTTTGCTATCGGTGATGTCCGCCAAAAAGATCTTCGTCAAATTACGA
CAGCTGTTGGCGATGGTGCCGTAGCAGCTCAAGAAGCCTATCAATATATT
GTCAATAACTATTAA

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