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Streptococcus mutans Search Results

Record: 1 of 1  
MiniMap IGR832 IGR829 IGR834 IGR828 IGR833 IGR831 IGR827 SMu0956 satC, - SMu0967 radC, - SMu0963 relA,yjbM, - SMu0955 satE, - SMu0965 rluE, - SMu0953 SMu0964 SMu0960 SMu0957 rex, - SMu0961 guaA, - SMu0962 prs,prsA, - SMu0958 nifS, - SMu0959 SMu0956 satC, - SMu0967 radC, - SMu0963 relA,yjbM, - SMu0955 satE, - SMu0965 rluE, - SMu0953 SMu0964 SMu0960 SMu0957 rex, - SMu0961 guaA, - SMu0962 prs,prsA, - SMu0958 nifS, - SMu0959 SMu0956 radC, - SMu0963 relA,yjbM, - SMu0955 satE, - SMu0965 rluE, - SMu0953 satD, - SMu0966 ppnK, - SMu0954 satD, - SMu0966 ppnK, - SMu0954 satC, - SMu0967 SMu0964 SMu0960 SMu0957 rex, - SMu0961 guaA, - SMu0962 prs,prsA, - SMu0958 nifS, - SMu0959


LANL Gene ID: SMu0959

GenBank Locus Tag: SMU.1051

DNA Molecule Name:
1  

GenBank ID:
24379488

Gene Name:
nifS  

Definition:
aminotransferase (class V); possible iron-sulfur cofactor synthesis protein;pyridoxal-phosphate dependent aminotransferase

Cellular Location:
Cytoplasm [Evidence]

Gene Start:
997355

Gene Stop:
998416

Gene Length:
1062

Molecular Weight*:
39093

pI*:
6.60

Net Charge*:
-3.42

EC:
 

Functional Class:
Protein synthesis; Protein modification  

Gene Ontology:
Biological process
  GO:0008152    metabolic process

Molecular function
  GO:0003824    catalytic activity
  GO:0008483    transaminase activity
  GO:0030170    pyridoxal phosphate binding


Pathway: pathway table

Secondary Evidence:
Flint,D.H.
Escherichia coli contains a protein that is homologous in function
and N-terminal sequence to the protein encoded by the nifS gene of
Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase
J. Biol. Chem. 271 (27), 16068-16074 (1996)
PubMed: 8663056

Kambampati,R. and Lauhon,C.T.
IscS is a sulfurtransferase for the in vitro biosynthesis of
4-thiouridine in Escherichia coli tRNA
Biochemistry 38 (50), 16561-16568 (1999)
PubMed: 10600118

Mihara,H., Kurihara,T., Yoshimura,T. and Esaki,N.
Kinetic and mutational studies of three NifS homologs from
Escherichia coli: mechanistic difference between L-cysteine
desulfurase and L-selenocysteine lyase reactions
J. Biochem. 127 (4), 559-567 (2000)
PubMed: 10739946




Comment:
For other 'nif' genes see SMu0226 (nifS) and SMu0227 (nifU).



View in HOMD Genome Viewer

Blast Summary:  PSI-Blast Search
Several matches in gapped BLAST to iron-sulfur cofactor synthesis protein and to pyridoxal-phosphate dependent aminotransferase: residues 1-352 are 64% similar to the protein in S.pyogenes (gi15675102) and are 59% similar to pyridoxal-phosphate dependent aminotransferase from S.pneumoniae (gi15903045).





The best hit to the Streptococcus agalactiae 2603 V/R genome is to SAG1098 (1e-136).


Top Blast Hits:  Updated monthly
Click here to view the entire PsiBlast results.
 gi|24379488|ref|NP_721443.1|  putative iron-sulfur cofactor ...   688   0.0  
 gi|25011216|ref|NP_735611.1|  hypothetical protein gbs1165 [...   473   e-132
 gi|77410465|ref|ZP_00786826.1|  cysteine desulphurase [Strep...   473   e-132
 gi|76787090|ref|YP_329804.1|  cysteine desulfurase, putative...   472   e-131
 gi|22537256|ref|NP_688107.1|  cysteine desulphurase [Strepto...   471   e-131
 gi|55821445|ref|YP_139887.1|  aminotransferase (class V), pu...   445   e-123
 gi|116628165|ref|YP_820784.1|  Cysteine sulfinate desulfinas...   443   e-123
 gi|15675102|ref|NP_269276.1|  putative iron-sulfur cofactor ...   438   e-121
 gi|139473785|ref|YP_001128501.1|  putative cysteine desulfur...   438   e-121
 gi|50914188|ref|YP_060160.1|  Cysteine desulfhydrase [Strept...   437   e-121


InterPro Summary:  InterProScan

InterPro
IPR000192
Family
Aminotransferase, class V
PF00266 [9-343]T 2.599982339102901E-72 PF00266 Aminotran_5 Aminotran_5
PS00595 [172-191]T 8.0E-5 PS00595 AA_TRANSFER_CLASS_5 AA_TRANSFER_CLASS_5
InterPro
IPR015421
Domain
Pyridoxal phosphate-dependent transferase, major region, subdomain 1
G3DSA:3.40.640.10 [1-230]T 2.500009099162E-72 G3DSA:3.40.640.10 PyrdxlP-dep_Trfase_major_sub1 PyrdxlP-dep_Trfase_major_sub1
InterPro
IPR015424
Domain
Pyridoxal phosphate-dependent transferase, major region
SSF53383 [1-353]T 7.0E-91 SSF53383 PyrdxlP-dep_Trfase_major PyrdxlP-dep_Trfase_major
noIPR
unintegrated
unintegrated
PIRSF005572 [1-353]T 1.19999063421838E-104 PIRSF005572 NifS NifS
PTHR11601 [1-351]T 1.19999063421921E-89 PTHR11601 PTHR11601 PTHR11601


COGS Summary:  COGS Search
BeTs to 13 clades of COG1104
COG name: Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes (NifS family)
Functional Class: E
The phylogenetic pattern of COG1104 is A--kYQvCeBRHuj---lINX
Number of proteins in this genome belonging to this COG is 2
Blocks Summary:  Blocks Search
No significant hits to the Blocks database.

 ProDom Summary:  Protein Domain Search
Residues 28-194 are 54% similar to a (AMINOTRANSFERASE PHOSPHATE PYRIDOXAL COMPLETE PROTEOME) protein domain (PD000087 which is seen in O34599_BACSU.

Residues 19-85 are 47% similar to a (AMINOTRANSFERASE PROTEOME PYRIDOXAL LYASE) protein domain (PD311278 which is seen in ISCS_PSEAE.

Residues 267-320 are 70% similar to a (LYASE PYRIDOXAL DESULFURASE PHOSPHATE 4.4.1.- CYSTEINE) protein domain (PD189309 which is seen in Q9CEI2_LACLA.

Residues 94-187 are 41% similar to a (AMINOTRANSFERASE V PROTEOME COMPLETE) protein domain (PD393971 which is seen in Q9RXU1_DEIRA.

Residues 189-228 are 60% similar to a (LYASE PHOSPHATE 4.4.1.- PYRIDOXAL) protein domain (PD001054 which is seen in ISCS_METTE.


Paralogs:  Local Blast Search
SMu0959 is paralogously related (blast p-value < 1e-3) to SMu0765, a predicted aminotransferase and SMu0226, a predicted NifS protein homolog, class-V aminotransferase.


Pfam Summary:  Pfam Search
Residues 1 to 343 (E-value = 1.7e-81) place SMu0959 in the Aminotran_5 family which is described as Aminotransferase class-V (PF00266)

 Top PDB Hits:
pdb1ECXB Chain B, Nifs-Like Protein >gi7546479pdb1ECXA Ch... 228 1e-060
pdb1EG5B Chain B, Nifs-Like Protein >gi7546280pdb1EG5A Ch... 221 1e-058
pdb1C0NA Chain A, Csdb Protein, Nifs Homologue 95 1e-020

Gene Protein Sequence:
MTDTMTSVFGNPSSIHTYGRTANKVLRERRQQVAQIFDVKSRNIIFTSGG
TESNNTAIKGYALANQFKGKHIISTSIEHHSVLHTLDYLEQRFGFEVTYL
NPIKGKILMQQVKDALRSDTILVSIMFANNETGDILPIQEIGNVIKEHQA
VFHVDAVQTVGKLPIHPKELNIDFLSASAHKFHGPKGVGILYVADNLHFD
NLLHGGEQEEKRRASTENLIGIVGLTQALKDSYLHQENVLNYIDGLKKLF
LDELEGLDYYLNSNEDALPHVINIGFPNYNNSILLTQLDLAGFAISTGSA
CTAGTVDPSHVLIAKYGLDSVRLQESIRISFSDLNTPDEVKALAVKIKEI
IGK

Gene Nucleotide Sequence:  Sequence Viewer
ATGACAGACACGATGACTTCTGTTTTTGGCAATCCTTCTAGTATTCATAC
TTATGGTAGAACGGCTAATAAGGTCTTAAGAGAGCGTCGTCAACAAGTTG
CTCAGATTTTTGATGTAAAATCGCGAAATATTATTTTTACTTCTGGTGGA
ACTGAGAGCAATAATACTGCCATTAAAGGTTATGCTCTAGCAAATCAGTT
CAAAGGTAAACACATTATCTCAACTAGCATTGAACATCATTCTGTTTTGC
ATACTCTTGATTACTTAGAACAACGTTTTGGTTTTGAAGTGACTTACCTT
AATCCTATCAAAGGTAAAATTCTTATGCAACAAGTTAAAGACGCTTTGCG
ATCTGATACGATTTTAGTATCTATAATGTTTGCTAATAATGAAACAGGAG
ATATATTGCCAATTCAAGAAATCGGCAATGTGATTAAAGAACATCAGGCA
GTTTTTCACGTTGATGCTGTTCAAACAGTTGGCAAACTGCCTATTCACCC
TAAAGAACTAAACATTGATTTTCTTTCCGCTTCTGCTCATAAATTTCATG
GTCCAAAAGGGGTAGGGATTCTGTATGTTGCTGATAATCTTCATTTTGAT
AATCTGCTTCATGGGGGTGAGCAAGAAGAAAAACGGCGTGCCAGTACAGA
AAATCTCATCGGAATTGTTGGTCTTACTCAAGCTTTAAAGGATAGTTATC
TTCATCAGGAAAATGTTTTAAACTATATTGACGGACTCAAAAAGTTATTC
CTAGATGAATTGGAAGGTTTAGATTACTATCTTAATTCTAATGAAGATGC
TCTTCCTCATGTTATTAATATTGGTTTCCCTAATTATAATAATAGTATTT
TACTGACACAATTAGATCTAGCAGGATTTGCAATTTCAACTGGTTCCGCT
TGTACGGCTGGTACTGTTGATCCCAGTCATGTCTTGATAGCAAAATATGG
ATTAGATTCTGTACGGCTTCAAGAGTCAATACGTATCAGTTTTTCAGACT
TGAATACTCCAGATGAGGTTAAGGCATTAGCAGTAAAAATTAAAGAAATT
ATAGGAAAATAA

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