Age-Related Changes in Myosin Heavy Chain Composition in Human Orbicularis Oculi Muscle - Tabular View

This Tabular View shows the required WHO registration data elements as marked by ^†

Tracking Information

First Received Date ^†

September 9, 2005

Last Updated Date

September 9, 2005

Start Date ^†

January 2004

Current Primary Outcome Measures ^†

Differentiation capability of preadipocyte

Original Primary Outcome Measures ^†

Same as current

Change History

No Changes Posted

Current Secondary Outcome Measures ^†

Proliferation capability of preadipocyte

Original Secondary Outcome Measures ^†

Same as current

Descriptive Information

Brief Title ^†

Age-Related Changes in Myosin Heavy Chain Composition in Human Orbicularis Oculi Muscle

Official Title ^†

Brief Summary

Orbicularis oculi muscle is a key structure in the peri-orbital anatomy and is responsible for the major changes of aging in this area. Peri-orbital rejuvenation has been an issue of great interest in plastic surgery. We believe that understanding the age-related histomorphometric changes in orbicularis oculi muscle is important for improving the current rejuvenation techniques or developing the new ones. Nevertheless, a study focusing on this topic has never been performed before, simply because it is difficult and dangerous to perform needle biopsy on orbicularis oculi muscle.

Detailed Description

Hundreds of people came to our hospital requesting upper blepharoplasty every year. Taking advantage of the discarded soft tissue in the blepharoplasty surgery, samples of bilateral orbicularis oculi muscle can be easily obtained from patients of various ages. This study is designed to objectively evaluate the age-related changes in human orbicularis oculi muscle by analyzing the composition of isoforms of myosin heavy chain (MHC), which is the predominant protein in skeletal muscle. MHC isoforms exist in different types of muscle fibers, and determines the maximum contraction speed of the muscle fiber. Therefore, The composition of MHC isoforms represents the composition of different types of muscle fiber.

These muscle specimens are stored with liquid nitrogen right after surgery. In the lab, the specimens are sectioned to 40μm in thickness, and soaked in special buffer solution. After electrophoresis and staining, bands of different types of MHC can be visualized. The gel is scanned and processed with an image analysis system. The density of each band can be determined, and the ratio of the different types of MHC can be calculated.

By dividing the subjects who had their orbicularis oculi muscle sampled during upper blepharoplasty operation into age groups of 20, 30, 40, 50, 60 and 70 years, these MHC subtypes composition ratios can be compared among the different groups. Therefore, the age-related changes of the composition of different muscle types can be concluded.

Study Phase

Phase I

Study Type ^†

Interventional

Study Design ^†

Educational/Counseling/Training, Non-Randomized, Open Label, Uncontrolled, Single Group Assignment, Efficacy Study

Condition ^†

Aging

Intervention ^†

Procedure: obtain surgical specimen for analysis

Study Arms / Comparison Groups

Publications *

* Includes publications given by the data provider as well as publications identified by National Clinical Trials Identifier (NCT ID) in Medline.

Recruitment Information

Recruitment Status ^†

Completed

Enrollment ^†

Completion Date

August 2004

Primary Completion Date

Eligibility Criteria ^†

Inclusion Criteria:

patients receiving blepharoplasty

Exclusion Criteria:

Gender

Both

Ages

20 Years to 80 Years

Accepts Healthy Volunteers

Yes

Contacts ^††

Location Countries ^†

Expanded Access Status

Administrative Information

NCT ID ^†

NCT00154453

Responsible Party

Secondary IDs ^††

Study Sponsor ^†

National Taiwan University Hospital

Collaborators ^††

Investigators ^†

Principal Investigator:

Yueh-Bih Tang, M.D. Ph.D.

National Taiwan University Hospital

Information Provided By

National Taiwan University Hospital

Verification Date

October 2003

^† Required WHO trial registration data element.
^†† WHO trial registration data element that is required only if it exists.