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Takeshi Ohe,¹ Nobue Takeuchi,¹ Tetsushi Watanabe,² Atsuko Tada,³ Haruo Nukaya,⁴ Yoshiyasu Terao,⁵ Hiroyuki Sawanishi,⁶ Teruhisa Hirayama,² Takashi Sugimura,³ and Keiji Wakabayashi³

¹Department of Food and Nutrition Science, Kyoto Women's University, Kitahiyoshi-cho, Imakumano, Higashiyama-ku, Kyoto, Japan
²Department of Public Health, Kyoto Pharmaceutical University, 5-Nakauchi-cho, Misasagi, Yamashina-ku, Kyoto, Japan
³Cancer Prevention Division, National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan
⁴School of Pharmaceutical Science; ⁵Graduate School of Nutrition and Environmental Sciences, University of Shizuoka, Yada, Shizuoka, Japan
⁶Faculty of Pharmaceutical Sciences, Hokuriku University, Kanagawa-machi, Kanazawa, Japan

Abstract

The levels of two aromatic amine mutagens, 2-[2-(acetylamino) -4-[bis(2-methoxyethyl) amino]- 5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-1) and 2-[2-(acetylamino-4-[N-(2-cyanoethyl) ethylamino]- 5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2H-benzotriazole (PBTA-2) , were quantitatively analyzed in the Yodo River system in Japan. The river water samples were collected at nine sampling sites from the Yodo River system twice or three times between May and July in 1997. PBTA-1 and PBTA-2 in the river water samples were concentrated on blue rayon columns, partially purified by high-performance liquid chromatography (HPLC) on reverse-phase columns, then quantified by HPLC with an electrochemical detector. The amounts of PBTA-1 and PBTA-2 in the water samples were < 0.01-1.91 and < 0.01-2.25 ng/L, respectively. High levels of PBTA-1 and PBTA-2 were detected in the samples collected within 4 km downstream of two sewage plants, which are located along the banks of the Nishitakase River, a tributary of the Yodo River system, and these samples showed stronger mutagenicity in Salmonella typhimurium YG1024 with S9 mix than the other water samples. On the other hand, the river water samples from upstream of the sewage plant were weakly or not mutagenic and PBTA-1 and PBTA-2 were not detected. These results confirmed that a major source of PBTA-1 and PBTA-2 in the Yodo River system is effluent from the sewage plants and that discharged mutagens, including PBTA-1 and PBTA-2, are diluted and/or decomposed while moving down the Yodo River system. Key words: mutagenicity, PBTA-1, PBTA-2, quantification, river water. Environ Health Perspect 107:701-704 (1999) . [Online 27 July 1999]

http://ehpnet1.niehs.nih.gov/docs/1999/107p701-704ohe/ abstract.html

Address correspondence to T. Ohe, Department of Food and Nutrition Science, Kyoto Women's University, Kitahiyoshi-cho, Imakumano, Higashiyama-ku, Kyoto 605-8501 Japan. Telephone: 075 531 7155. Fax: 075 531 7216. E-mail: oeosaka@oak.ocn.ne.jp

This study was supported by Grants-in-Aid for Cancer Research from the Ministry of Health and Welfare of Japan and funds under a contract with the Environment Agency of Japan.

Received 23 February 1999 ; accepted 5 May 1999.