| Progress in the Application of DNA Microarrays Edward K. Lobenhofer, Pierre R. Bushel, Cynthia A. Afshari, and Hisham K. Hamadeh Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA Abstract
Microarray technology has been applied to a variety of different fields to address fundamental research questions. The use of microarrays, or DNA chips, to study the gene expression profiles of biologic samples began in 1995. Since that time, the fundamental concepts behind the chip, the technology required for making and using these chips, and the multitude of statistical tools for analyzing the data have been extensively reviewed. For this reason, the focus of this review will be not on the technology itself but on the application of microarrays as a research tool and the future challenges of the field. Key words: DNA chips, gene expression profiling, microarray, toxicogenomics. Environ Health Perspect 109:881-891 (2001) . [Online 16 August 2001] http://ehpnet1.niehs.nih.gov/docs/2001/109p881-891lobenhofer/ abstract.html Address correspondence to C.A. Afshari, National Institute of Environmental Health Sciences, P.O. Box 12233, Research Triangle Park, NC 27709 USA. Telephone: (919) 541-1310. Fax: (919) 541-0146. E-mail: afshari@niehs.nih.gov We thank L.T. Bennett, C.J. Tucker, R. Amin, J.B. Collins, G.A. Boorman, and T.R. Devereux for critically reviewing this manuscript as well as providing insightful comments. Received 4 January 2001 ; accepted 12 March 2001.
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