Johns Hopkins University
Hopkins Digestive Diseases Basic Research Development Center (DDBRDC)
Director: Mark Donowitz, M.D.
Co-Directors: A Hubbard, R. Cole, B. Guggino, S. Guggino, O. Kovbasnjuk, S. Leach
Center Focus The Hopkins Digestive Diseases Basic Research Development Center is designed to advance basic science and translational digestive diseases research at the Johns Hopkins University School of Medicine and to foster collaborations and new directions of digestive (gastrointestinal and liver) diseases research by providing access for its 26 members and associate members to three Cores: Epithelial Proteomics, Imaging and Mouse Physiology. The Center is a partnership between the JHUSOM and the members of this Center, with investment in personnel and equipment by the JHUSOM supplementing budget. Cores help to identify interactions among proteins including identification of binding partners, examine protein turnover, map cleavage sites and determine post-translational modifications. The approaches use 2D separations that are specific for hydrophobic, membrane proteins as well as mass spectrometry. This Core will provide a technician to work out 2D separation techniques. Also, several types of cutting edge imaging and EM of epithelial cells are available to DDBRDC investigators including confocal and two-photon microscopy; microfluorometer/cooled CCD camera digitizing system; and constant temperature/O2/CO2 microscope stage interfaced with camera/confocal microscope for study of the same cells in culture for hours-days. Investigators, also have assistance in the preparation of intestine, liver, pancreas and kidney for EM. In addition, investigators will be able to genotype mouse models of digestive diseases, use metabolic cages and perform urine, stool, blood collections and analysis as well as perform aortic perfusion of mice as part of intestinal. Liver, pancreas, kidney procurement and preparation for Northern and Western analysis. This approach will also be used to remove, orient, fix, process embed. section and mount these tissues to be used for light microscopy as well as provide instruction in the use of Ussing chamber/voltage clamp/active intestinal NA and CI transport studies.
- Epithelial Proteomics
- Mouse Physiology
Page last updated: November 25, 2008