Electronic PCR (e-PCR) is computational procedure that is used to identify sequence tagged sites(STSs), within DNA sequences. e-PCR looks for potential STSs in DNA sequences by searching for subsequences that closely match the PCR primers and have the correct order, orientation, and spacing that could represent the PCR primers used to generate known STSs.

What's new
Improved Search Sensitivity

The new version of e-PCR implements a fuzzy matching strategy.To reduce likelihood that a true STS will be missed due to mismatches, multiple discontigous words may be used instead of a single exact word. Each of this word has groups of significant positions seperated by 'wildcard' positions that are not required to match. In addition, it is also possible to allow gaps in the primer alignments.

Reverse Searching

The main motivation for implementing reverse searching (called Reverse e-PCR) was to make it feasible to search the human genome sequence and other large genomes. The new version of e-PCR provides a search mode using a query sequence against a sequence database.

   Schuler,GD. (1997)

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Related Resources
UniSTS is a NCBI resource that reports information about markers or Sequence Tagged Sites (STSs).
Map Viewer allows you to view and search an organism's complete genome, display chromosome maps, and zoom into greater levels of detail.
The whole genomes of several eukaryotes, over 1000 viruses and 100 microbes can be found in Entrez Genome.
UniGene is a system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters.
BLAST(Basic Local Alignment Search Tool), provides a method for rapid searching of nucleotide and protein databases.






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