Electronic PCR (e-PCR) is computational procedure that is used to identify sequence tagged sites(STSs), within DNA sequences.
e-PCR looks for potential STSs in DNA sequences by
searching for subsequences that closely
match the PCR primers and have the correct
order, orientation, and spacing that could represent the PCR primers used to generate known STSs.
Improved Search Sensitivity
The new version of e-PCR implements a fuzzy matching strategy.To reduce likelihood that a true STS will be missed
due to mismatches, multiple discontigous words may be used instead of a single exact word. Each of this word has groups of
significant positions seperated by 'wildcard' positions that are not required to match. In addition, it is also possible to
allow gaps in the primer alignments.
The main motivation for implementing reverse searching (called Reverse e-PCR) was to make it feasible to search the human genome
sequence and other large genomes. The new version of e-PCR provides a search mode using a query sequence against a sequence
UniSTS is a NCBI resource that reports information about markers or Sequence Tagged Sites (STSs).
Map Viewer allows you to view and search an organism's complete genome, display chromosome maps,
and zoom into greater levels of detail.
The whole genomes of several eukaryotes, over 1000 viruses and 100 microbes can be found in Entrez Genome.
UniGene is a system for automatically partitioning GenBank sequences into a non-redundant set of gene-oriented clusters.
BLAST(Basic Local Alignment Search Tool), provides a method for rapid searching of nucleotide and protein databases.