Laboratory Analytes and Repository Plan - Health ABC

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	Examination 1 N=32121	Examination 2 N=27341	Examination 3 N=27341	Examination 4 N=2850	Examination 5 N=2800	Examination 6 N=2800	Examination 7 N=2800
Stored specimens:
	1 extracted DNA, microtiter plates	cells for later transformation
	19 aliquots serum2	10 aliquots serum	4 serum	4 serum		10 serum
	12 aliquots EDTA plasma3	10 aliquots EDTA	4 citrated	7 plasma		10 plasma
	4 aliquots citrated plasma5	plasma	plasma*
	3 aliquots urine4	4 aliquots citrated plasma  1 Vitamin C aliquot 4 urine aliquots	Cells for later transformation* platelets*
		Platelets frozen from citrated plasma for mitochondrial DNA	*plasma & platelets only collected from those who had cells collected in Year 3 (plasma sent to LCBR for pilot studies)
Total specimens for storage in each examination:	139,087	94,238	12,101	12,000		11,400
1N includes 5% blind duplicates 2Aliquots = 13 aliquots 0.5 ml, 6 aliquots 1 ml. 3Aliquots = 8 aliquots 0.5 ml, 4 aliquots 1 ml. 4Aliquots = 2 ml, 1 acidified, 2 not. 5Aliquots = 0.5 ml
Analytes Obtained:	Examination 1	Examination 2	Examination 3	Examination 4	Examination 5	Examination 6	Examination 7
	Fasting glucose, insulin, two-hour (after glucola); glycated hemoglobin	Fasting glucose TSH, Free T-4*	Non-fasting, CBC Arterialized venous pH	Fasting glucose, glycated hemoglobin		Fasting glucose, glycated hemoglobin  Total cholesterol, HDL-cholesterol, triglycerides
	Total cholesterol, HDL- and LDL- cholesterol, triglycerides, creatinine			Total cholesterol
	Albumin, alkaline phosphatase	* in those with TSH alert
Analytes added:	Examination 1	Examination 2	Examination 3	Examination 4	Examination 5	Examination 6	Examination 7
	Serum IL-6, TNF-a, CRP, leptin, PAI-1, Oxidized LDL
Analytes proposed:	Examination 1	Examination 2	Examination 3	Examination 4	Examination 5	Examination 6	Examination 7
	Selected polymorphisms: ACE, IL-6, TNF-a, PPAR-8, RxR, HSP70-2, PAI-1 Selected musculoskeletal genes

Page last updated Sep 26, 2008