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FBI Laboratory Specialized Training Program

Forensic Mitochondrial DNA Analysis

Course dates to be announced

This two-week course is presented for crime laboratory examiners and biologists conducting or preparing to conduct forensic mitochondrial DNA (mtDNA) analysis. The course provides classroom and laboratory experience in the principles and procedures involved in typing mtDNA from evidentiary items such as hair, teeth, and bones.

Classroom instruction is focused on the nature of mtDNA, molecular biology principles involved in the polymerase chain reaction (PCR), sequencing, and legal issues related to this technique. Discussions regarding scientific literature published in the area of forensic mtDNA analysis will be held. Laboratory procedures include DNA extraction, PCR, quantification of amplified DNA by capillary electrophoresis, and automated sequencing. Computer-based practice compiling sequences and database searches using appropriate software is provided. Moot court exercises are conducted.

FBI Laboratory Training Application (pdf)
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