RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Dolphin ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Coryphaena hippurus
FDA Seafood List Common Name: Dolphin
FDA Seafood List Market Name: Mahi Mahi
Unique IEF Gel Code: 19990426gel01
Unique Web Code: mhg03Srr
Tissue Aliquot Code: coryhipp J98-001 #2 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	57	2.6	1.70918	6	15.9106	11.7	10	3.5	57	5.6	1.59187
2	4.55	354	16.3	0.334083	10	5.87309	4.3	3.7	4.55	352.8	9.7	0.342653
3	5.2	519	23.9	0.999649	11	19.6103	14.4	12.4	5.2	523.9	11.1	0.996879
4	5.85	816	37.6	1.39482	10	24.3298	17.9	15.3	5.85	818.7	9.9	1.38995
5	6.55	1172	54	1.82839	4	12.2488	9	7.7	6.55	1172.8	4.1	1.69983
6	6.85	1246	57.4	0.096796	20	3.06646	2.3	1.9	6.85	1248.6	20.3	0.0852541
7	7.35	1421	65.4	2.40068	6	26.4448	19.5	16.7	7.35	1422.9	6.4	2.31885
8	8.15	1719	79.1	0.150187	19	4.93811	3.6	3.1	8.15	1719.4	19.3	0.144374
9	8.45	1842	84.8	0.283128	11	5.45784	4	3.4	8.45	1842.2	11.2	0.275164
10	8.65	1915	88.2	0.234072	9	3.63236	2.7	2.3	8.65	1914.3	8.9	0.229189
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2145	98.8	2.30494	4	14.243	10.5	9	9.3	2144	3.8	2.12601

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 114
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.62	362	16.7	0.0441305	10	0.697956	0.2	0.2	4.62	363.1	10.2	0.0386442
2	4.888	431	19.9	0.0484764	11	0.954935	0.3	0.3	4.888	429	11.2	0.0481821
3	5.02	465	21.4	0.0396951	11	0.697977	0.2	0.2	5.02	465.9	10.6	0.0370438
4	5.214	518	23.9	0.0415052	13	0.865768	0.3	0.3	5.214	521.9	12.7	0.0385936
5	5.331	572	26.3	0.0922337	5	0.809217	0.3	0.2	5.331	572.4	5.3	0.0864665
6	5.37	590	27.2	0.062646	8	0.897027	0.3	0.3	5.37	588.3	8.4	0.0599142
7	5.508	654	30.1	0.0257888	7	0.338009	0.1	0.1	5.508	655.9	7.3	0.0261385
8	5.623	707	32.6	0.0833699	6	0.882817	0.3	0.3	5.623	706.5	6.5	0.0770158
9	5.738	760	35	0.24117	5	1.67908	0.6	0.5	5.738	759.6	4.6	0.206563
10	5.757	769	35.4	0.493634	5	4.50863	1.5	1.4	5.757	768.3	5.3	0.482279
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.88	827	38.1	0.0247528	12	0.509323	0.2	0.2	5.88	831	11.7	0.024665
12	5.983	878	40.4	0.0507532	4	0.387821	0.1	0.1	5.983	877.5	4.3	0.0512904
13	6.015	894	41.2	0.0207267	4	0.131795	0	0	6.015	893.9	3.7	0.0203582
14	6.105	939	43.3	0.0453699	5	0.374839	0.1	0.1	6.105	939.3	4.9	0.0429354
15	6.168	970	44.7	0.0845735	9	1.32139	0.4	0.4	6.168	970.2	9.2	0.0813551
16	6.313	1042	48	2.35822	5	24.5521	8.1	7.5	6.313	1044.3	5.2	2.67441
17	6.391	1081	49.8	0.238544	5	2.01535	0.7	0.6	6.391	1079.7	5.1	0.224427
18	6.409	1090	50.2	2.32464	4	15.764	5.2	4.8	6.409	1090.3	3.9	2.29436
19	6.455	1113	51.3	0.0805537	4	0.633551	0.2	0.2	6.455	1113	4.5	0.0798254
20	6.484	1127	51.9	0.0596168	8	0.803528	0.3	0.2	6.484	1127.2	7.6	0.0592777
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.516	1143	52.6	0.0712644	7	0.850152	0.3	0.3	6.516	1143	6.7	0.0716075
22	6.581	1168	53.8	0.059164	4	0.41221	0.1	0.1	6.581	1168.1	3.9	0.0603625
23	6.686	1195	55	0.137887	10	2.14534	0.7	0.7	6.686	1195.1	10.1	0.12021
24	6.718	1203	55.4	0.165973	5	0.861099	0.3	0.3	6.718	1204.9	4.6	0.105451
25	6.799	1224	56.4	1.17516	3	6.84413	2.3	2.1	6.799	1224.5	3.1	1.23723
26	7.134	1337	61.6	0.0630999	9	1.20163	0.4	0.4	7.134	1340.6	8.9	0.0759957
27	7.18	1353	62.3	0.160064	5	1.19545	0.4	0.4	7.18	1352.8	4.6	0.147242
28	7.248	1377	63.4	0.0678715	8	0.839603	0.3	0.3	7.248	1376.6	7.8	0.0609491
29	7.35	1413	65.1	0.0284311	7	0.341985	0.1	0.1	7.35	1411.5	7.4	0.0262503
30	7.425	1441	66.4	2.3886	5	23.4415	7.8	7.2	7.425	1438	4.9	2.69611
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.647	1524	70.2	0.360059	14	8.80919	2.9	2.7	7.647	1523.4	14.1	0.351463
32	7.961	1641	75.6	2.36254	5	22.5039	7.5	6.9	7.961	1643.3	4.8	2.6685
33	8.122	1701	78.4	0.300323	26	13.326	4.4	4.1	8.122	1699.4	25.5	0.294447
34	8.488	1849	85.2	2.27041	24	94.8405	31.4	29.2	8.488	1854.5	23.6	2.26857
35	8.593	1888	87	2.17634	20	64.5274	21.4	19.8	8.593	1892.5	20.3	1.79506
36	8.813	1967	90.6	0.0475795	10	0.757946	0.3	0.2	8.813	1969.2	9.6	0.0447348

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	82	3.7	0.543006	21	18.9123	15.3	13.1	3.5	77.6	20.9	0.511445
2	4.55	352	16.1	0.755325	5	6.45434	5.2	4.5	4.55	353.5	5.3	0.686544
3	5.2	522	23.8	2.40066	5	19.5933	15.9	13.6	5.2	521	5	2.22925
4	5.85	826	37.7	2.40584	4	19.8754	16.1	13.8	5.85	824.2	4.2	2.63971
5	6.55	1166	53.3	1.56356	4	9.4346	7.6	6.5	6.55	1165.9	3.6	1.47886
6	6.85	1246	56.9	0.0959121	15	2.31631	1.9	1.6	6.85	1244.5	15.1	0.0867317
7	7.35	1423	65	2.40467	4	15.2494	12.4	10.6	7.35	1423.5	3.7	2.31339
8	8.15	1722	78.7	0.177366	16	5.08646	4.1	3.5	8.15	1722.4	16.3	0.176524
9	8.45	1846	84.3	0.287483	11	5.48417	4.4	3.8	8.45	1846.3	11.1	0.279493
10	8.65	1921	87.8	0.303418	8	4.12197	3.3	2.9	8.65	1920.9	7.9	0.295188
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2154	98.4	2.32741	4	16.8213	13.6	11.7	9.3	2153.8	3.9	2.44372

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	99	4.5	0.420258	22	14.8157	4.3	4.1	3.5	95.7	21.6	0.386888
2	4.451	339	15.4	0.0179951	5	0.167198	0	0	4.451	338.4	5.4	0.0173452
3	4.55	364	16.6	0.408348	5	2.45254	0.7	0.7	4.55	364.4	5	0.2751
4	4.664	393	17.9	0.0587928	30	2.63998	0.8	0.7	4.664	365.5	30	0.0496492
5	4.805	429	19.5	0.0225584	5	0.158964	0	0	4.805	429.4	5.1	0.017555
6	4.949	466	21.2	0.029753	7	0.321755	0.1	0.1	4.949	466.9	6.5	0.0278891
7	5.2	530	24.1	1.15887	5	8.67537	2.5	2.4	5.2	528.8	4.8	1.01356
8	5.247	552	25.1	0.279384	14	5.87249	1.7	1.6	5.247	545.5	14.1	0.234804
9	5.289	572	26	0.0697995	11	1.3136	0.4	0.4	5.289	578.3	10.6	0.0701603
10	5.484	664	30.2	0.0215521	14	0.483113	0.1	0.1	5.484	660.1	14.1	0.0193033
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.556	698	31.8	0.0664245	12	1.32046	0.4	0.4	5.556	701.5	11.9	0.0627871
12	5.647	741	33.7	0.0826662	8	1.1301	0.3	0.3	5.647	742.2	8.4	0.0763191
13	5.702	767	34.9	0.243033	12	4.90934	1.4	1.4	5.702	768.7	12.1	0.228655
14	5.85	837	38.1	1.39879	8	16.9845	4.9	4.8	5.85	838.5	8	1.1951
15	5.956	886	40.3	0.0341429	8	0.481772	0.1	0.1	5.956	886.7	8.3	0.0326821
16	6.083	945	43	0.0370284	17	1.13003	0.3	0.3	6.083	950.9	16.9	0.0376602
17	6.175	988	45	0.0783851	9	1.20746	0.3	0.3	6.175	987.2	8.7	0.07835
18	6.313	1052	47.9	0.000203684	1	0	0	0	6.313	1050	1	0
19	6.35	1069	48.7	2.18932	10	47.24	13.6	13.2	6.35	1070.7	10.3	2.58161
20	6.41	1097	50	0.170923	2	0.350969	0.1	0.1	6.41	1099	2.1	0.093655
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.432	1107	50.4	2.21213	3	11.2973	3.3	3.2	6.432	1106.8	2.6	2.40913
22	6.449	1115	50.8	0.876108	3	3.93457	1.1	1.1	6.449	1115.6	2.7	0.815571
23	6.479	1129	51.4	0.133936	3	0.749617	0.2	0.2	6.479	1129.5	3.1	0.137019
24	6.513	1145	52.1	0.103421	11	3.43509	1	1	6.513	1155.6	11.4	0.170656
25	6.55	1162	52.9	0.979366	3	4.62195	1.3	1.3	6.55	1162.3	2.9	0.885999
26	6.638	1188	54.1	0.110957	3	0.554797	0.2	0.2	6.638	1188	2.8	0.111202
27	6.715	1211	55.1	0.0943548	5	0.681516	0.2	0.2	6.715	1210	4.7	0.0820909
28	6.776	1229	56	0.221749	9	2.67035	0.8	0.7	6.776	1230.5	8.9	0.168523
29	6.847	1250	56.9	2.35048	3	12.2274	3.5	3.4	6.847	1248.7	2.7	2.58022
30	7.155	1354	61.7	0.0649794	10	1.09168	0.3	0.3	7.155	1358.1	9.9	0.0624505
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.217	1375	62.6	0.147684	5	1.22773	0.4	0.3	7.217	1375.1	5.1	0.13561
32	7.35	1420	64.7	1.00594	5	7.96417	2.3	2.2	7.35	1419.3	5.1	0.889709
33	7.402	1440	65.6	0.0288024	4	0.205731	0.1	0.1	7.402	1440.4	3.9	0.0296112
34	7.469	1466	66.8	2.38452	5	19.9011	5.7	5.6	7.469	1465.1	4.7	2.40148
35	7.685	1550	70.6	0.208063	18	6.41708	1.9	1.8	7.685	1547.4	17.8	0.20296
36	8.039	1687	76.8	1.46392	5	9.86745	2.8	2.8	8.039	1686.2	5.5	1.02145
37	8.15	1730	78.8	0.433285	26	18.5474	5.4	5.2	8.15	1729.7	25.9	0.40358
38	8.45	1847	84.1	1.1036	9	17.2191	5	4.8	8.45	1849.3	8.5	1.13651
39	8.558	1897	86.4	1.1444	29	58.3329	16.8	16.3	8.558	1897.3	29.1	1.13092
40	8.65	1940	88.3	1.64898	14	36.7659	10.6	10.3	8.65	1933.7	14.3	1.45447
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
41	8.788	1987	90.5	0.0555153	5	0.426163	0.1	0.1	8.788	1987.6	5.3	0.0457377
42	9.3	2162	98.5	2.3601	4	16.721	4.8	4.7	9.3	2163	4.3	2.17818

PeakCounter: 42

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.687	380	17.4	0.0383756	11	0.652701	0.2	0.2	4.687	381.4	10.7	0.0342641
2	4.958	450	20.6	0.0379507	11	0.713906	0.2	0.2	4.958	449.1	10.9	0.0370434
3	5.136	496	22.8	0.0138891	8	0.347536	0.1	0.1	5.136	489.7	7.9	0.0248956
4	5.254	538	24.7	0.0512075	16	1.46627	0.5	0.4	5.254	541.5	16.2	0.0510741
5	5.328	573	26.3	0.0401736	9	0.70861	0.2	0.2	5.328	571.5	9.5	0.0422455
6	5.387	601	27.6	0.0695231	12	1.20421	0.4	0.4	5.387	599.8	12.1	0.0560755
7	5.508	658	30.2	0.0211261	13	0.469286	0.2	0.1	5.508	658	12.5	0.0211416
8	5.62	711	32.6	0.0682859	7	0.771109	0.3	0.2	5.62	710.9	6.8	0.0643986
9	5.705	751	34.4	0.197279	5	1.61739	0.5	0.5	5.705	750.1	4.7	0.196014
10	5.756	775	35.6	0.429887	5	3.52902	1.1	1.1	5.756	774.8	4.8	0.414014
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.896	841	38.6	0.0202817	9	0.29309	0.1	0.1	5.896	839.6	8.8	0.0187498
12	5.973	877	40.2	0.0389642	6	0.365626	0.1	0.1	5.973	877.5	5.5	0.0372909
13	6.089	931	42.7	0.0288671	9	0.492191	0.2	0.1	6.089	934.5	9.5	0.0293295
14	6.177	972	44.6	0.0597199	7	0.825553	0.3	0.2	6.177	970.7	7.4	0.062521
15	6.311	1034	47.4	0.0644625	10	0.264651	0.1	0.1	6.311	1034.9	10.1	0.0148168
16	6.341	1048	48.1	2.29471	9	39.3671	12.8	11.7	6.341	1048.1	9.3	2.38908
17	6.399	1075	49.3	0.0934406	6	0.690915	0.2	0.2	6.399	1078.2	5.7	0.0686378
18	6.416	1083	49.7	0.75003	3	3.60924	1.2	1.1	6.416	1082.6	3	0.671341
19	6.433	1091	50	0.352054	3	2.1403	0.7	0.6	6.433	1090.3	3.4	0.358548
20	6.461	1104	50.6	0.090104	3	0.510009	0.2	0.2	6.461	1103.8	3	0.0946953
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.493	1119	51.3	0.0708818	5	0.589853	0.2	0.2	6.493	1119.7	4.8	0.0692731
22	6.604	1161	53.3	0.0912243	3	0.504453	0.2	0.2	6.604	1160.7	3.2	0.0890631
23	6.678	1182	54.2	0.0686499	7	0.770463	0.3	0.2	6.678	1178.8	7.1	0.0615269
24	6.724	1195	54.8	0.12294	6	1.26023	0.4	0.4	6.724	1197.4	5.6	0.126479
25	6.811	1220	56	2.37347	2	10.0739	3.3	3	6.811	1219.8	2.2	2.55788
26	7.123	1325	60.8	0.0670937	9	1.0522	0.3	0.3	7.123	1328.1	9.1	0.0655784
27	7.181	1345	61.7	0.121945	5	0.998764	0.3	0.3	7.181	1344.4	4.9	0.114224
28	7.26	1372	62.9	0.0379618	10	0.546726	0.2	0.2	7.26	1372.8	9.7	0.0317713
29	7.371	1411	64.7	0.0432991	7	0.50177	0.2	0.1	7.371	1410.1	7.3	0.0387316
30	7.438	1437	65.9	2.39408	4	19.9137	6.5	5.9	7.438	1435.9	4.4	2.54311
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.641	1515	69.5	0.170289	17	5.14751	1.7	1.5	7.641	1511.5	17.4	0.167014
32	7.985	1647	75.6	0.32918	9	5.32183	1.7	1.6	7.985	1647.5	9.3	0.321897
33	8.123	1700	78	0.182887	29	8.98855	2.9	2.7	8.123	1701.7	28.7	0.176631
34	8.504	1852	85	2.28246	26	98.1108	31.9	29.2	8.504	1851.1	25.7	2.1557
35	8.615	1899	87.1	2.37385	20	80.3507	26.1	24	8.615	1894.5	20.1	2.25728
36	8.789	1963	90	0.21758	30	13.6693	4.4	4.1	8.789	1946.7	30	0.257074

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	88	4	1.52656	10	24.7379	17.1	14.5	3.5	87.9	10.3	1.35908
2	4.55	350	16	1.10211	6	10.1641	7	5.9	4.55	352.1	6.3	0.908189
3	5.2	520	23.8	1.88992	7	22.0477	15.2	12.9	5.2	520.3	6.7	1.85259
4	5.85	827	37.8	2.40654	4	19.5263	13.5	11.4	5.85	827.6	4.4	2.48467
5	6.55	1154	52.8	2.28078	3	10.6917	7.4	6.3	6.55	1154.2	2.6	2.30113
6	6.85	1236	56.6	0.150923	13	2.90874	2	1.7	6.85	1235.7	12.7	0.12904
7	7.35	1411	64.6	2.38942	4	17.1371	11.8	10	7.35	1412.3	4	2.4117
8	8.15	1716	78.5	0.193554	17	5.54706	3.8	3.2	8.15	1715.4	16.7	0.187287
9	8.45	1837	84.1	0.426668	10	7.59749	5.2	4.4	8.45	1837.5	10.4	0.410937
10	8.65	1912	87.5	0.416621	7	5.25222	3.6	3.1	8.65	1911.5	7.5	0.397593
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2153	98.5	2.36495	4	19.4782	13.4	11.4	9.3	2153.9	4.2	2.59764

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 147

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 11.700 4.550 4.300 5.200 14.400 5.850 17.900 6.550 9.000 6.850 2.300 7.350 19.500 8.150 3.600 8.450 4.000 8.650 2.700 9.300 10.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
4.620 0.200 4.888 0.300 5.020 0.200 5.214 0.300 5.331 0.300 5.370 0.300 5.508 0.100 5.623 0.300 5.738 0.600 5.757 1.500 5.880 0.200 5.983 0.100 6.015 0 6.105 0.100 6.168 0.400 6.313 8.100 6.391 0.700 6.409 5.200 6.455 0.200 6.484 0.300 6.516 0.300 6.581 0.100 6.686 0.700 6.718 0.300 6.799 2.300 7.134 0.400 7.180 0.400 7.248 0.300 7.350 0.100 7.425 7.800 7.647 2.900 7.961 7.500 8.122 4.400 8.488 31.400 8.593 21.400 8.813 0.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 114 PeakCount: 36 Return to Lane Navigator
3.500 15.300 4.550 5.200 5.200 15.900 5.850 16.100 6.550 7.600 6.850 1.900 7.350 12.400 8.150 4.100 8.450 4.400 8.650 3.300 9.300 13.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 4.300 4.451 0 4.550 0.700 4.664 0.800 4.805 0 4.949 0.100 5.200 2.500 5.247 1.700 5.289 0.400 5.484 0.100 5.556 0.400 5.647 0.300 5.702 1.400 5.850 4.900 5.956 0.100 6.083 0.300 6.175 0.300 6.313 0 6.350 13.600 6.410 0.100 6.432 3.300 6.449 1.100 6.479 0.200 6.513 1.000 6.550 1.300 6.638 0.200 6.715 0.200 6.776 0.800 6.847 3.500 7.155 0.300 7.217 0.400 7.350 2.300 7.402 0.100 7.469 5.700 7.685 1.900 8.039 2.800 8.150 5.400 8.450 5.000 8.558 16.800 8.650 10.600 8.788 0.100 9.300 4.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 42 Return to Lane Navigator
4.687 0.200 4.958 0.200 5.136 0.100 5.254 0.500 5.328 0.200 5.387 0.400 5.508 0.200 5.620 0.300 5.705 0.500 5.756 1.100 5.896 0.100 5.973 0.100 6.089 0.200 6.177 0.300 6.311 0.100 6.341 12.800 6.399 0.200 6.416 1.200 6.433 0.700 6.461 0.200 6.493 0.200 6.604 0.200 6.678 0.300 6.724 0.400 6.811 3.300 7.123 0.300 7.181 0.300 7.260 0.200 7.371 0.200 7.438 6.500 7.641 1.700 7.985 1.700 8.123 2.900 8.504 31.900 8.615 26.100 8.789 4.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 36 Return to Lane Navigator
3.500 17.100 4.550 7.000 5.200 15.200 5.850 13.500 6.550 7.400 6.850 2.000 7.350 11.800 8.150 3.800 8.450 5.200 8.650 3.600 9.300 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Dolphin ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Dolphin ---- Go to Display Gel Lane Navigator.