National Toxicology Program

CASRN: 320-67-2

Formula: C8 H12 N4 O5

Synonyms/Common Names:

• 4-AMINO-1-BETA-D-RIBOFURANOSYL-1,3,5-TRIAZIN-2(1H)-ONE

Known Uses:

ANTICANCER DRUG AND ANTIBIOTIC

Short-Term Toxicity

• 14-Day (Intraperitoneal Injection)  (C01569) Completed 
  • NTIS No. PB279526  (Peer Review 11/28/1977)
    Citation: Bioassay of 5-Azacytidine for Possible Carcinogenicity (CAS No. 320-67-2)
  • Rats: Sprague Dawley; Mice: B6C3F1
• 13-Week (Intraperitoneal Injection)  (C01569) Completed 
  • NTIS No. PB279526  (Peer Review 11/28/1977)
    Citation: Bioassay of 5-Azacytidine for Possible Carcinogenicity (CAS No. 320-67-2)
  • Rats: Sprague Dawley; Mice: B6C3F1
  • Dose: FR: 0,0.13,2.6, MM: 0,.22,4.4, FM: 0,4.4,35.2 MG/KG/5 PER GROUP

Long-Term Carcinogenicity

• 2-Year (Intraperitoneal Injection)  (C01569) Report Complete 
  • TR-042 (NTIS No. PB279526)  (Peer Review 11/28/1977)
    Bioassay of 5-Azacytidine for Possible Carcinogenicity (CAS No. 320-67-2)
  • Rats: Sprague Dawley; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Inadequate Study 
  • Female Rats Inadequate Study 
  • Male Mice Inadequate Study 
  • Female Mice Positive 
  • Dose: R: 0,2.6,5.2, M: 0,2.2,4.4 MG/KG/35 PER GROUP

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (932806) Completed 
  • Chromosome Aberation Positive 
  • Sister Chromatid Exchange Positive 
• Mouse Lymphoma  (010439) Completed 
  •  Positive 
• Micronucleus  (A95392) Completed 
  • Mice: B6C3F1
  • Male Positive 
• Salmonella  (440974) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelman, K. Salmonella Mutagenicity Tests V. Results from the Testing of 311 Chemicals Environ. Molec. Mutagen. Vol. 19 (Suppl 21) (1992) 2-141
  •  Positive 
• Salmonella  (A21960) Completed 
  •  Positive 

Organ Systems Toxicity

• Total Reproductive Capacity  (TRC98004) Completed 
  • Citation: Bishop, JB, Morris, RW, Seely, JC, Hughes, LA, Cain, KT, Generoso, WM. Alterations in the Reproductive Patterns ofFemale Mice Exposed to Xenobiotics. Fund. and Appl. Tox. 40: 191-204 (1997).
  • F Mice
  • Mice Female Negative