National Toxicology Program

CASRN: 2475-45-8

Formula: C14-H12-N4-O2

Synonyms/Common Names:

• 1,4,5,8-TETRAAMINO-9,10-ANTHRACENEDIONE (9CI)
• 1,4,5,8-TETRAAMINOANTHRAQUINONE

Known Uses:

DYE FOR SHEEPSKINS, FURS, THERMOPLASTIC RESINS, ACETATE, NYLON (HSDB 1990); ALSO A COMPONENT OF SEVERAL SEMIPERMANENT HAIR DYES (TR-299, 1986)

Short-Term Toxicity

• 14-Day (Dosed-Feed)  (C54900) Completed 
  • NTIS No. PB86-248051  (Peer Review 03/29/1985)
    Citation: Toxicology and Carcinogenesis Studies of C.I. Disperse Blue 1 (A commercial dye containing approximately 50% 1,4,5,8-tetraaminoanthraquinone, and 20% water) (CAS No. 2475-45-8) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Dosed-Feed)  (C54900) Completed 
  • NTIS No. PB86-248051  (Peer Review 03/29/1985)
    Citation: Toxicology and Carcinogenesis Studies of C.I. Disperse Blue 1 (A commercial dye containing approximately 50% 1,4,5,8-tetraaminoanthraquinone, and 20% water) (CAS No. 2475-45-8) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,1200,20000, M: 0,600,10000 PPM/10 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C54900) Report Complete 
  • TR-299 (NTIS No. PB86-248051)  (Peer Review 03/29/1985)
    Toxicology and Carcinogenesis Studies of C.I. Disperse Blue 1 (A commercial dye containing approximately 50% 1,4,5,8-tetraaminoanthraquinone, and 20% water) (CAS No. 2475-45-8) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Clear Evidence 
  • Female Rats Clear Evidence 
  • Male Mice Equivocal Evidence 
  • Female Mice No Evidence 
  • Dose: R: 0,12500,2500,5000, M: 0,600,1200,2500 PPM/50 PER GROUP

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (776658) Completed 
  • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
  • Chromosome Aberation Positive 
  • Sister Chromatid Exchange Positive 
• Mouse Lymphoma  (334618) Completed 
  • Citation: Myhr B.C., McGregor, D., Bowers, L., Riach, C., Brown, A., Edwards, I., McBride, D., Martin, R., and Caspary, W.J. L5178Y mouse lymphoma cell mutation assay results with 41 compounds. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 138-167
  •  Positive 
• Micronucleus  (A40953) Completed 
  • Mice: B6C3F1
  • Male Negative 
• Salmonella  (336679) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelmans, K. Salmonella mutagenicity tests. IV. Results from the testing of 300 chemicals Environ. Molec. Mutagen. Vol. 11 (Suppl 12) (1988) 1-158
  •  Positive