National Toxicology Program

CASRN: 84-66-2

Formula: C12-H14-O4

Synonyms/Common Names:

• 1,2-BENZENEDICARBOXYLIC ACID DIETHYL ESTER
• DEP

Known Uses:

IN MANUFACTURE OF CELLULOID, COSMETICS, VARNISHES, AND DOPES. PLASTICIZER FOR CELLULOSE ESTER PLASTICS. INSECTICIDE SPRAYS. (TDB)

Nomination Background

Short-Term Toxicity

• 4-WEEK (Topical Application)  (C60048B) Completed 
  • NTIS No. PB96-162276  (Peer Review 11/17/1993)
    Citation: Toxicology and Carcinogenesis Studies of Diethylphthalate (CAS No. 84-66-2) in F344/N Rats and B6C3F1 Mice (Dermal Studies) with Dermal Initiation/Promotion Study of Diethylphthalate and Dimethylphthalate (CAS No. 131-11-3) in Male Swiss (CD-1®) Mice
  • Rats: F344/N; Mice: B6C3F1

Long-Term Carcinogenicity

• 2-YEAR (Topical Application)  (C60048B) Report Complete 
  • TR-429 (NTIS No. PB96-162276)  (Peer Review 11/17/1993)
    Toxicology and Carcinogenesis Studies of Diethylphthalate (CAS No. 84-66-2) in F344/N Rats and B6C3F1 Mice (Dermal Studies) with Dermal Initiation/Promotion Study of Diethylphthalate and Dimethylphthalate (CAS No. 131-11-3) in Male Swiss (CD-1®) Mice
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats No Evidence 
  • Female Rats No Evidence 
  • Male Mice Equivocal Evidence 
  • Female Mice Equivocal Evidence 
  • Dose: R: 0,100,300 M: 0,7.5,15,30 UL/100 UL SOLUTION /50 PER GROUP

Special Studies

• Absorption Disposition Metabolism Elimination (Topical Application)  (S0044) Completed 
  • M Rats: F344

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (306322) Completed 
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Positive 
• In Vitro Cytogenetics (CA/SCE)  (913646) Completed 
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Positive 
• Salmonella  (364986) Completed 
  • Citation: Zeiger, E., Haworth, S., Mortelmans, K., and Speck, W. Mutagenicity testing of Di(2-ethylhexyl)phthalate and related chemicals in Salmonella Environ. Mutagen. Vol. 7 (1985) 213-232
  •  Negative 
• Salmonella  (576172) Completed 
  • Citation: Zeiger, E., Haworth, S., Mortelmans, K., and Speck, W. Mutagenicity testing of Di(2-ethylhexyl)phthalate and related chemicals in Salmonella Environ. Mutagen. Vol. 7 (1985) 213-232
  •  Negative 

Organ Systems Toxicity

• CONT EXPOS, 120 D MINIMUM Continuous Breeding (Dosed-Feed)  (RACB83092) Completed 
  • NTIS No. PB85-118636
    Citation: Final Report - Diethyl Phthalate: Reproduction and Fertility Assessment In CD-1 Mice When Administered in the Feed
  • M/F Mice: Swiss
  • Dose: N/A; 0,.25,1.25,2.5% IN FEED; N/A; 0,2.5%
• GD 7-14 Short-Term in vivo Repro. Tox. (Gavage)  (T0033A) Completed 
  • NTIS No. PB85-220143
    Citation: Screening of Priority Chemicals for Potential Reproductive Hazard
  • F Mice: Swiss
  • Dose: 4500 MG/KG BODY WEIGHT
• GD 6-15 Conventional Teratology (Dosed-Feed)  (TER88065) Completed 
  • NTIS No. PB89-140081
    Citation: Developmental Toxicity Evaluation of Diethyl Phthalate Administered to CD Rats on Gestational Days 6 through 15?
  • F Rats: Sprague Dawley
  • Rats Maternal Positive 
  • Rats Fetal Positive 
  • Dose: 0.25, 2.5, 5.0 %