The Expression Arrest™ microRNA-adapted shRNA (shRNAmir) libraries were developed in collaboration with Greg Hannon (CSHL) and Steve Elledge (Harvard). shRNAmir triggers for mammalian RNAi are based on current knowledge of the endogenous microRNA biogenesis pathway. shRNAmir constructs are designed to mimic a natural microRNA primary transcript, enabling specific processing by the endogenous RNAi pathway and producing more effective knockdown (Silva et al 2005). Genome wide shRNAmir libraries incorporate several features aimed at increasing the efficiency and specificity of gene knockdown providing solutions for diverse RNAi applications. These include:
- microRNA-30 adapted design for increased knockdown specificity and efficiency
- Transient, stable and in vivo options for RNAi
- Pre-cloned into retroviral and lentiviral vectors
- Human and mouse genomes targeted
- Guaranteed knockdown
Available shRNAmir libraries targeting the human and/or mouse whole genomes include:
Individual constructs, target gene sets, RNAintro starter kits, gene families/pathway sets, custom rearrays, whole genome libraries are available.
This product is covered by the following limited use licenses:Limited Use License-shRNA technologies
Limited Use License-Tet Technology
Limited Use License-ddRNAi
TurboGFP and TurboRFP are covered by a Limited Use License from Evrogen.
