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Mass Spectrometry Group Allison N. Schorzman, Ph.D.
IRTA Fellow Tel (919) 316-4596 Fax (919) 541-7880 schorzmana@niehs.nih.gov P.O. Box 12233 Mail Drop F0-11 Research Triangle Park, North Carolina 27709 Delivery Instructions My research focuses on the application of protein footprinting techniques coupled with mass spectrometry to characterize protein-ligand interactions. In my current investigation, I am applying differential oxidative surface mapping to characterize the DNA-binding site of the N-terminal domain of the yeast mismatch repair protein, Pms1-NTD. The radiolysis of water generates hydroxyl radicals that can oxidize solvent accessible side chains of the protein. The experiment is performed in the presence and absence of DNA. Through mass spectrometric analysis of Pms1-NTD proteolytic peptides, the rates of oxidation can be compared where a reduced rate of oxidation indicates interaction with DNA. Along with the known x-ray crystal structure of Pms1-NTD, the data from these experiments will allow us to construct a model for the interaction of Pms1 with dsDNA. Allison Null Schorzman, Ph.D., is an IRTA postdoctoral fellow in the Mass Spectrometry Group within the Laboratory of Structural Biology. She received her B.A. in Biology from the University of Virginia in 1995 and her Ph.D. in Chemistry from Virginia Commonwealth University in 2003. |
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