U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M163
Tryptone Salt Agar
(T1N1 Agar and T1N2 Agar)

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Trypticase or tryptone 10 g
NaCl 10 g
Agar 20 g
Distilled water 1 liter

Suspend ingredients and boil to dissolve agar. For slants, dispense into tubes. Autoclave 15 min at 121°C. Solidify tubes as slants. Cool medium for plates to 45-50°C and pour into sterile petri dishes.

For T1N2 agar, use 20 g NaCl rather than the 10 g specified for T1N1 agar.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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