U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R11
Butterfield's Phosphate-Buffered Dilution Water

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Stock solution

KH2PO4 34 g
Distilled water 500 ml

Adjust pH to 7.2 with 1 N NaOH. Bring volume to 1 liter with distilled water. Sterilize 15 min at 121°C. Store in refrigerator.



Dilution blanks

Take 1.25 ml of above stock solution and bring volume to 1 liter with distilled water. Dispense into bottles to 90 or 99 ± 1 ml. Sterilize 15 min at 121°C.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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