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Validation Study of In Vitro Cytotoxicity Test Methods
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Provided by:
Dr. Richard Clothier from the FRAME Alternatives Laboratory, University of Nottingham, UK.

NHK-NRU low magnification

Neutral red uptake in normal human keratinocyte cells after treatment with Sodium Lauryl Sulfate (low magnification)
NHK-NRU high magnification

Neutral red uptake in normal human keratinocyte cells after treatment with Sodium Lauryl Sulfate (high magnification)
NHK-NRU high magnification

Standard plate setup for the 3T3 and NHK neutral red uptake assays. VC = vehicle control; C1 = highest concentration of test compound; C8 = lowest concentration of test compound; Rows A and H, columns 1 and 12 are blank wells without cells. Sodium Lauryl Sulfate with 3T3 mouse cells.
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