Emerging Fluorescence Technologies for Analysis of Protein Localization and Organelle Dynamics |
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| Air date: | Wednesday, April 04, 2007, 3:00:00 PM |
| Category: | Wednesday Afternoon Lectures |
| Runtime: | 75 minutes |
| NLM Title: | Emerging fluorescence technologies for analysis of protein localization and organelle dynamics [electronic resource] / Jennifer Lippincott-Schwartz. |
| Series: | NIH director's Wednesday afternoon lecture series |
| Author: | Lippincott-Schwartz, Jennifer. National Institutes of Health (U.S.) |
| Publisher: | [Bethesda, Md. : National Institutes of Health, 2007] |
| Other Title(s): | NIH director's Wednesday afternoon lecture series |
| Abstract: | (CIT): The development of fluorescent proteins as molecular tags over the past decade has splurred a revolution by allowing complex biochemical processes to be correlated with the functioning of proteins in livings cells. Fluorescent proteins such as green fluorescent protein (GFP) from the jelly fish Aequorea Victoria and its variants can be fused to virtually any protein of interest to analyze protein geography, movement and chemistry in living cells. As such, they have provided an important new tool for understanding protein function, filling an urgent need now that the genome sequence of many organisms is complete. Among the new fluorescent proteins are those that can be photoactivated. These molecules are invisible until activated by a specific wavelength of light, at which point they become brightly fluorescent. I will discuss results from two new techniques employing photoactivatable fluorescent proteins: in cellula pulse-chase analysis and photoactivated localization microscopy (PALM). |
| Subjects: | Luminescent Proteins Microscopy, Fluorescence--methods Organelles Proteins--analysis Proteins--metabolism |
| Publication Types: | Government Publications Lectures |
| Download: | Download
Video How to download a Videocast |
| NLM Classification: | QU 55 |
| NLM ID: | 101306056 |
| CIT File ID: | 13738 |
| CIT Live ID: | 5195 |
| Permanent link: | http://videocast.nih.gov/launch.asp?13738 |
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Enhanced Audio Podcast | 1:08:01 |
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Enhanced Video Podcast | 1:08:01 | |