Harlan Animal Colonies Health Evaluation Summary: 8/28/06
Contract N01-AG-9-2105
RATS Barrier 236A
| Animal Facility |
Strain |
No.Sex |
Age |
Testing Laboratory/Dates |
| Location: Oregon, WI |
BN/RijHsd |
0M/0F |
|
University of Missouri |
| Barrier/Building: 236A |
|
2M/2F |
10 months |
UMRADIL, Columbia, MO |
| |
F344 x BN F1 |
1M/1F |
5 months |
|
| |
|
2M/2F |
10 months |
Receipt Date: 6/12/06 |
| Ship Date: 6/11/06 |
|
|
|
Report Date: 7/26/06 |
Evaluation
| NIA rats submitted for this health monitoring were obtained from NIA-NIH aging colonies maintained in barriers by Harlan Sprague dawley, Inc. All rats examined were considered to be in a good state of health. |
Serology
| Agent |
Assay |
No. Pos./No. Tested |
Comments |
| Sendai |
MFI |
0/10 |
|
| RCV/SDAV |
MFI |
0/10 |
|
| Parvoviruses |
MFI |
0/10 |
|
| KRV |
MFI |
0/10 |
|
| H-1 |
MFI |
0/10 |
|
| PVM |
MFI |
0/10 |
|
| M. pulmonis |
MFI |
0/10 |
|
| Reo 3 |
MFI |
0/10 |
|
| TMEV GDVII |
MFI |
0/4 |
|
| MAD |
MFI |
0/4 |
|
| LCM |
MFI |
0/4 |
|
| E. cuniculli |
MFI |
0/4 |
|
| Hantaan |
MFI |
0/4 |
|
| Tyzzer's |
MFI |
0/4 |
|
Bacteriology
| Agent |
No. Pos./No. Tested |
Comments |
| Salmonella spp. |
0/10 |
Nasopharynx/cecum cultures |
| Pasturella pneumotropica |
0/10 |
|
| Pasturella spp. |
0/10 |
|
| Bordetella bronchiseptica |
0/10 |
|
| Pseudomonas aeruginosa |
0/10 |
|
| Citrobacter rodentium |
0/10 |
|
| Corynebaterium kutscheri |
0/10 |
|
| Klebsiella pneumoniae |
2/10 |
|
| Klebsiella oxytoca |
0/10 |
The bacteria isolated from the oropharynx and cecum are opportunistic or normal flora of the rat. |
| Staphylococcus aureus |
0/10 |
| Streptococcus sp. Group B beta |
0/10 |
|
PCR
| |
No. Pos./No.Tested |
Comments |
| Mycoplasma pulmonis |
0/10 |
|
| Heliobacter spp |
0/10 |
|
Parasitology
| Type and Test |
Results |
Comments |
| Ectoparasites |
No. Pos./No. Tested |
External parasites were not observed in any rat. |
Test: Direct pelage |
0/10 |
| Endoparasites |
No. Pos./No. Tested |
Enteric and Urinary bladder parasites were not observed in any rat. |
Test(s): Direct, Gross, and Microscopic (intestine & bladder) |
0/10 |
Pathology
| Gross: |
None Significant |
Overall frequency of lesions in these rats is low. |
| Histologic: |
|
|
| Lung lymphoid hyperplasia |
9/10 |
Lesions are incidental and/or consistent with normal aging process and are not indicative of infection with murine adventitious pathogens. |
| Nephrocalcinosis |
1/10 |
| Hydroneophrosis |
2/10 |
|
A few incidental lesions were also noted. |
|
|
Availability
|
Rats obtained from NIA-NIH aging colonies maintained in one maximum security barrier by Harlan Sprague Dawley, Inc., (Harlan) in Oregon, Wisconsin. The combined census of these colonies, at the time of the referenced monitoring is tabulated below:
|
Virgin Male (1-37 months of age) |
7,584 |
|
Virgin Female (1-33 months of age)
|
950
|
|
Total Census: |
8,534 |
|
For information, contact:
Dr. Robert J. Russell
Harlan Sprague Dawley, Inc.
PO Box 29176
Indianapolis, IN 46229
Phone: (317) 894-7521
Fax: (317) 894-4473
Signed by Robert J. Russell, D.V.M., Principal Investigator
Page last updated Sep 26, 2008
