Harlan Animal Colonies Health Evaluation Summary: 2/22/08
Contract N01-AG-9-2105
RATS Barrier 208A
| Animal Facility |
Strain |
No./Sex |
Age |
Testing Laboratory/Dates |
| Location: Frederick, MD |
BN/RijHsd |
2M/3F |
12 months |
University of Missouri |
| Barrier/Building: 208A |
|
|
|
UMRADIL, Columbia, MO |
| |
F344 x BN F1 |
3M/2F |
11 months |
|
| |
|
|
|
Receipt Date: 12/10/07 |
| Ship Date: 12/9/07 |
|
|
|
Report Date: 2/12/08 |
Evaluation
| NIA rats submitted for this health monitoring were obtained from NIA-NIH aging colonies maintained in barriers by Harlan Sprague Dawley, Inc. All rats examined were considered to be in a good state of health. |
Serology
| Agent |
Assay |
No. Pos./No. Tested |
Comments |
| Sendai |
MFI |
0/10 |
|
| RCV/SDAV |
MFI |
0/10 |
|
| Parvoviruses |
MFI |
0/10 |
|
| KRV |
MFI |
0/10 |
|
| H-1 |
MFI |
0/10 |
|
| PVM |
MFI |
0/10 |
|
| M. pulmonis |
MFI |
0/10 |
|
| Reo 3 |
MFI |
0/10 |
|
| TMEV GDVII |
MFI |
0/3 |
|
| MAD |
MFI |
0/3 |
|
| LCM |
MFI |
0/3 |
|
| E. cuniculli |
MFI |
0/3 |
|
| Hantaan |
MFI |
0/3 |
|
| Tyzzer's |
MFI |
0/3 |
|
Bacteriology
| Agent |
No. Pos./No. Tested |
Comments |
| Salmonella spp. |
0/10 |
Nasopharynx/cecum cultures |
| Pasturella pneumotropica |
0/10 |
|
| Pasturella spp. |
0/10 |
|
| Bordetella bronchiseptica |
0/10 |
|
| Pseudomonas aeruginosa |
0/10 |
|
| Citrobacter rodentium |
0/10 |
|
| Corynebaterium kutscheri |
0/10 |
|
| Klebsiella pneumoniae |
0/10 |
|
| Klebsiella oxytoca |
0/10 |
The bacteria isolated from the oropharynx and cecum are opportunistic or normal flora of the rat. |
| Staphylococcus aureus |
0/10 |
| Streptococcus sp. Group B beta |
0/10 |
|
PCR
| |
No. Pos./No.Tested |
Comments |
| Mycoplasma pulmonis |
0/10 |
|
| Heliobacter spp |
0/10 |
|
Parasitology
| Type and Test |
Results |
Comments |
| Ectoparasites |
No. Pos./No. Tested |
External parasites were not observed in any rat. |
Test: Direct pelage |
0/10 |
| Endoparasites |
No. Pos./No. Tested |
Enteric and Urinary bladder parasites were not observed in any rat. |
Test(s): Direct, Gross, and Microscopic (intestine & bladder) |
0/10 |
Pathology
| Gross: |
None Significant |
Overall frequency of lesions in these rats is low. |
| Histologic: |
|
|
| Lymphoid hyperplasia - lung |
9/10 |
Lesions are incidental and/or consistent with normal aging process and are not indicative of infection with murine adventitious pathogens. |
| Hydronephrosis |
1/10 |
| Interstitial lymphoid infiltrate - Harderian gland |
1/10 |
| Nephrocalcinosis |
2/10 |
|
A few incidental lesions were also noted. |
|
|
Availability
|
Rats obtained from NIA-NIH aging colonies maintained in one maximum security barrier by Harlan Sprague Dawley, Inc., (Harlan) in Frederick, Maryland. The combined census of these colonies, at the time of the referenced monitoring is tabulated below:
|
Virgin Male (2-38 months of age) |
4,116 |
|
Virgin Female (5-39 months of age)
|
712
|
|
Total Census: |
4,828 |
|
For information, contact:
Dr. Karla Stevens
Harlan Sprague Dawley, Inc.
8520 Allison Pointe, #400
Indianapolis, IN 46250
Phone: (317) 806-6060
Fax: (317) 806-6090
Signed by Karla A. Stevens, D.V.M., Principal Investigator
Page last updated Sep 26, 2008
