shRNA Info

• shRNA Construct
• shRNA Vector Map
• pGIPZ Vector Map

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• Hannon Lab, CSH

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shRNA Construct

Design rationale

• miR30 flanking sequences of 125 nt on each end of the hairpin and the miR30 loop increase Drosha and Dicer processing of the expressed hairpin.
• shRNA clones contain 22 sense and 22 anti-sense nucleotide base pairs. The following criteria were used to select sequences:
  • Matched to only exonic (coding) regions of targeted gene
  • Contained >3 mismatches to any other gene
  • Where possible, shRNAs showed sequence identity between the human and mouse orthologue of the targeted gene