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Effects of Topical Anesthetic Pre-treatment on In Vivo Ocular Irritation Hazard Classification

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The Draize rabbit eye test has been in use since 1944 to evaluate the risk of substances to cause irritation or corrosion to the eyes. This test presents the potential for pain and distress in rabbits after application of a severely irritating or corrosive substance. Alternative approaches have been proposed and developed to reduce the number of animals used or refine (reduce pain and suffering) these tests. In October 2003, the U.S. Environmental Protection Agency requested that ICCVAM review ways to alleviate pain and suffering that might arise during current in vivo tests. The Scientific Advisory Committee on Alternative Toxicological Methods recommended that ICCVAM endorse this activity with a high priority. ICCVAM then recommended that NICEATM review the data currently available on the use of topical anesthetics and/or systemic analgesics to reduce animal pain and distress.

As part of this review, NICEATM has requested (Federal Register Notice, May 9, 2007) the submission of data from completed studies on the use of topical anesthetics and/or systemic analgesics for in vivo ocular irritancy testing. These data will be used to evaluate the validation status of the use of topical anesthetics and/or analgesics to reduce pain and distress for in vivo testing situations. ICCVAM and NICEATM also request the submission of information and data from in vivo methods, procedures, and/or strategies that may reduce or eliminate the pain and suffering associated with current in vivo eye irritation methods.


Federal Register Notice (Vol. 72, No. 89, page 26396, May 9, 2007):
Request for Data on the Use of Topical Anesthetics and Systemic Analgesics for In Vivo Eye Irritation Testing

View Federal Register Notice
View comments submitted on Federal Register Notice

NICEATM Presentations at 2007 Society of Toxicology and Sixth World Congress on Alternatives and Animal Use Meetings

Using data generated during testing of test substances to comply with regulatory requirements of governmental agencies, NICEATM staff conducted analyses evaluating of the effect of topical application of a tetracaine hydrochloride ophthalmic anesthetic solution on the irritancy potential of test substances evaluated using a sequential testing scheme. The results of these analyses were presented by NICEATM staff at two major scientific meetings in 2007.

View the NICEATM 2007 SOT Poster
View the NICEATM Sixth World Congress Poster

Abstract of platform presentation, "Alternatives for Ocular Toxicity Testing: ICCVAM and NICEATM Recent and Planned Initiatives", given by Dr. William Stokes at the Sixth World Congress

Symposium on Minimizing Pain and Distress in Ocular Toxicity Testing

In May 2005, ICCVAM, NICEATM, and the European Centre for the Validation of Alternative Methods (ECVAM), organized a Symposium on Minimizing Pain and Distress in Ocular Toxicity Testing. The symposium was sponsored by ICCVAM, NICEATM, ECVAM, and the European Cosmetic, Toiletry and Perfumery Association. The goals of this symposium were to:

  • Review current understanding of the sources and mechanisms of pain and distress in ocular toxicity testing
  • Identify current best practices for preventing, recognizing, and alleviating ocular pain and distress
  • Identify additional research, development, and validation studies necessary to support scientifically valid ocular toxicity testing procedures that avoid pain and distress
The participants in the symposium made the following recommendations:

  • Pre-treatment with topical or general anaesthesia should be used routinely to avoid pain from topical application of test substances
  • Systemic analgesics should be administered prior to test article application and continued until injuries resolve or the study is terminated
  • Ocular injuries predictive of severe or irreversible ocular damage should be used as earlier humane endpoints
  • Objective, quantitative measurements should be collected during ocular studies to assist in identifying earlier, more humane endpoints. Data from these measurements are also critical to the development and validation of more predictive in vitro methods.

View information about the Symposium


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