Recommendations for Future Studies
ICCVAM's recommendations on the use of in vitro test methods for detecting ocular corrosives and severe irritants
are provided in the ICCVAM Test Method Evaluation Report: In Vitro Test Methods for Identifying Ocular Corrosives
and Severe Irritants. The report includes ICCVAM’s general recommendations for all of the methods, and specific
recommendations for each of the four evaluated test methods. Several future studies were also recommended by ICCVAM which have subsequently been carried
forward as ICCVAM-nominated activities.
One of the nominated activities is an evaluation of the impact of protocol modifications on the accuracy and/or reliability of the Bovine Corneal Opacity
and Permeability (BCOP) test method. These include
use of an alternative corneal holder that maintains normal curvature (such as the corneal mounting system designed by Ubels et al. 2002), and
use of 0.9% sodium chloride instead of distilled water as the diluent.
View ICCVAM draft recommendations for evaluations of modifications of the BCOP test method
[PDF]
The remaining ICCVAM-nominated activities are more broadly applicable to in vitro ocular toxicity test methods and include:
- Creating a reference atlas of histopathology for chemically-induced ocular lesions.
- Creating a standardized scoring scheme to be used in conjunction with the histopathology atlas to identify decision criteria that could be used in hazard classification.
- Development of a targeted research grants program to focus on areas identified as high priority at the 2005 Symposium on Mechanisms of Chemically-Induced Ocular Injury and Recovery.
View ICCVAM recommendations for general activities applicable to in vitro ocular toxicity test methods
[PDF]
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