Volume 11, Number 2, February 2005

Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea

Yeon-Joo Choi,* Won-Jong Jang,* Jong-Hyun Kim,* Ji-Sun Ryu,* Seung-Hyun Lee,* Kyung-Hee Park,* Hyung-Suk Paik,† Young-Sang Koh,‡ Myung-Sik Choi,§ and Ik-Sang Kim§
*Konkuk University, Choongbuk, Republic of Korea; †Pusan National University, Pusan, Republic of Korea; ‡Cheju National University College of Medicine, Jeju, Republic of Korea; and §Seoul National University College of Medicine and Institute of Endemic Disease, Seoul, Republic of Korea

Figure 3. Restriction fragment length polymorphism analysis of H2-products amplified with multiplex-nested primer set from seropositive sera. Ethidium bromide–stained polyacrylamide gels of AluI restriction endonuclease digestion of ≈230-bp rickettsial DNA amplified by using the nested primer H set WJ77/80 in the primary reactions and WJ79/83/78 in the nested reactions. Lanes: M, size marker DNA (25-bp DNA ladder); 1, H3-2; 2, H7-2; 3, H8-2; 4, H13-2; 5, H14-2; 6, H15-2; 7, H18-2; 8, H19; P, Rickettsia prowazekii; T, R. typhi. P and T; predicted fragments after digestion. The number on the left indicates the molecular size (in base pairs) of restriction fragments.

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This page last reviewed January 21, 2005

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
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