| |
Figure. LightCycler amplification curves of the three single SARS-coronavirus
(CoV)-specific assays and the combined assay. Five hundred genome equivalents
(GE) of SARS-CoV were amplified with the three single assays-NS pp1a,
NS pp1ab, and SS GP-as well as with the combination of the three assays.
No fluorescence signal was observed for control pathogens. In the inset,
the respective agarose gel analysis of the amplification products in a
3% gel is shown. Lane 1: 50-bp marker, lane 2: 500 GE SARS-CoV amplified
with the assay combination (products of assays NS pp1ab and SS GP are
not clearly distinguishable because of sizes of 88 bp and 79 bp, respectively),
lane 3: human CoV 229E, lane 4: respiratory syncytial virus, lane 5: rhinovirus,
lane 6: human metapneumovirus, lane 7: Mycoplasma pneumoniae, or lane
8: Chlamydia pneumoniae.
|
