Solution 1

-Naphthol	5 g
Alcohol (absolute)	100 ml

Solution 2

Voges-Proskauer (VP) test

Transfer 1 ml of 48 h culture to test tube and add 0.6 ml solution 1 and 0.2 ml solution 2. Shake after adding each solution. To intensify and speed reaction, add a few creatine crystals to mixture. Let stand at room temperature. Read results 4 h after adding reagents.

Development of eosin pink color is positive test.

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