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EID
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Volume 11, Number 11, November 2005 Cryptococcus gattii in AIDS Patients, Southern CaliforniaSudha Chaturvedi,*† Madhu Dyavaiah,* Robert A. Larsen,‡§ and Vishnu
Chaturvedi*† |
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Figure 2. Multiplex polymerase chain reaction (PCR) for pheromone fragment analysis. A) Multiplex PCR with 4 sets of primers comprising MFα1 (V190/V191) and MFa1 (V290/V291, V1311/V1312, V1313/V1314) genes were carried out as described in Materials and Methods. Approximately 100-bp MFα1 and 117-bp MFa1 PCR amlicons were detected on 3.5% MetaPhor agarose in Tris-borate-EDTA buffer for MATα and MATa strains comprising Cryptococcus neoformans var. grubii (CnVG), Cryptococcus neoformans var. neoformans (CnVN), and Cryptococcus gattii (Cg). Lanes 1 and 7, molecular mass marker. B) Multiplex PCR depicting MFα1 and MFa1 PCR amplicons from the 8 known hybrid (A/D) isolates. Lane 1, molecular mass marker. |
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This page last reviewed October 13, 2005 |
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Emerging
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