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Dispatch
Isolation and Molecular Identification
of Nipah Virus from Pigs
Sazaly AbuBakar,*
Li-Yen Chang,* A.R. Mohd Ali,† S.H. Sharifah,† Khatijah Yusoff,‡ and Zulkeflie
Zamrod§
*University of Malaya, Kuala Lumpur, Malaysia; †Veterinary Research Institute,
Ipoh, Perak, Malaysia; ‡Universiti Putra Malaysia, Selangor, Malaysia;
and §Universiti Kebangsaan Malaysia, Selangor, Malaysia
Appendix
(a)
| Table.
Comparisons of the genome sequences of Nipah virus isolatesa |
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|
Gene:
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N
|
P
|
M
|
F
|
G
|
L
|
|
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|
|
|
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|
NV isolate
|
Nucleotide position
|
|
|
89
|
617
|
820
|
828
|
910
|
1132
|
2124
|
312
|
439
|
495
|
523
|
567
|
749
|
951
|
1017
|
1043
|
59
|
264
|
306
|
450
|
543
|
814
|
9
|
117
|
153
|
668
|
2226
|
2541
|
2704
|
3657
|
4714
|
4749
|
4934
|
5257
|
5268
|
6115
|
6486
|
6609
|
|
|
CDC
|
c
|
c
|
a
|
c
|
a
|
g
|
t
|
a
|
a
|
t
|
c
|
g
|
c
|
g
|
c
|
t
|
t
|
g
|
c
|
g
|
a
|
a
|
t
|
g
|
g
|
c
|
g
|
c
|
c
|
a
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c
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c
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c
|
a
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c
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c
|
a
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g
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|
UMMC1
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.
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UMMC2
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t
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NV-Flying
Fox
|
t
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t
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c
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t
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a
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.
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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UM-0128
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a
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t
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t
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NV-Tambun
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.
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.
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c
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t
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g
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a
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c
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g
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g
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c
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t
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a
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t
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a
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.
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.
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a
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a
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.
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g
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g
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c
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a
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a
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a
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t
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.
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g
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.
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.
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t
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g
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t
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a
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g
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a
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NV-Seremban
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t
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NV-Sungai
Buloh
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t
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a
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t
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(b)
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Gene:
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N
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P
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M
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F
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G
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L
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| NV isolate |
Amino acid position
|
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30
|
206
|
274
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276
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304
|
378
|
708
|
104
|
147
|
165
|
175
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189
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250
|
317
|
339
|
348
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20
|
88
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102
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150
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181
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272
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3
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39
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51
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223
|
742
|
847
|
902
|
1219
|
1572
|
1583
|
1645
|
1753
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1756
|
2039
|
2162
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2203
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|
CDC
|
T
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P
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S
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G
|
T
|
E
|
N
|
Q
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S
|
L
|
L
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K
|
T
|
L
|
I
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M
|
I
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Q
|
G
|
L
|
L
|
T
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D
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Q
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R
|
T
|
L
|
P
|
L
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P
|
L
|
H
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S
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M
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D
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H
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K
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K
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UMMC1
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F
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UMMC2
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NV-Flying
Fox
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I
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L
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T
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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UM-0128
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F
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NV-Tambun
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R
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A
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K
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G
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I
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N
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A
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N
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F
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V
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N
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.
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NV-Seremban
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NV-Sungai
Buloh
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F
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(c)
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Genome:
|
Nucleotide position
|
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|
NV isolate
|
201
|
295
|
520
|
529
|
1147
|
1669
|
1780
|
2035
|
2097
|
2215
|
2381
|
4952
|
6241
|
6461
|
8847
|
8864
|
8872
|
11124
|
11309
|
11315
|
18173
|
18180
|
|
|
CDC
|
c
|
t
|
a
|
c
|
g
|
a
|
g
|
a
|
c
|
g
|
c
|
t
|
t
|
a
|
a
|
t
|
g
|
t
|
a
|
g
|
t
|
t
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UMMC1
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UMMC2
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a
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NV-Flying
Fox
|
t
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t
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-
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-
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-
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-
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UM-0128
|
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NV-Tambun
|
.
|
c
|
g
|
a
|
a
|
g
|
t
|
c
|
a
|
a
|
.
|
a
|
c
|
c
|
g
|
c
|
a
|
c
|
t
|
.
|
c
|
g
|
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NV-Seremban
|
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NV-Sungai
Buloh
|
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| aResults shown are a summary
of all the nucleotides (a) and its corresponding deduced amino acids
(b) differences in the NV gene coding regions and nucleotides differences
in the NV noncoding regions (c) of the NV-Tambun, NV-Seremban, NV-Sungai
Buloh, and UM-0128 in comparison to other known NV isolates. |
|
