Volume 9, Number 1, January 2003

Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli

Catalina López-Saucedo,* Jorge F. Cerna,* Nicolas Villegas-Sepulveda,* Rocío Thompson,* F. Raul Velazquez,† Javier Torres,† Phillip I. Tarr,‡§ and Teresa Estrada-García*
*Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México D.F., México; †Hospital de Pediatría, México D.F., México; ‡Children’s Hospital and Regional Medical Center, Seattle, Washington, USA; and §University of Washington School of Medicine, Seattle, Washington, USA

Figure. Polymerase chain reaction (PCR) products of each locus. Lane 1: sizes of the seven PCR products of each locus in base pairs, obtained when using a DNA mix of the four reference strains and the primers mix. PCR products obtained by using DNA of enterotoxigenic Escherichia coli, Shiga-toxin–producing E. coli, enteropathogenic E. coli, and enteroinvasive E. coli (lanes 2–5, respectively). Lane 6–11: PCR products obtained when using DNA of patients’ isolates and the primers mix. Lanes 12–15: PCR products obtained when using DNA of food isolates and the primers mix. Lane 16: 1 kb molecular weight marker in base pairs.

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This page last reviewed March 20, 2003

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention