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EID
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Volume 12, Number 2, February 2006 Evaluation of a Direct, Rapid Immunohistochemical Test for Rabies DiagnosisTiziana Lembo,* Michael Niezgoda,† Andrés Velasco-Villa,† Sarah Cleaveland,*
Eblate Ernest,‡ and Charles E. Rupprecht† |
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Figure 1. Touch impression of a rabies-positive Tanzanian domestic dog brain preserved in 50% glycerol saline solution for 15 months before testing by direct rapid immunohistochemical test (dRIT) and retested by direct fluorescent-antibody assay (DFA) after 5 months. A) Brain stained by dRIT: rabies virus antigen appears as magenta inclusions (arrowheads) against the blue neuronal hematoxylin counterstain. Magnification, ×630. B) Immunofluorescent apple-green viral inclusions in the same brain processed by DFA. Magnification, ×200. |
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