ARE II2 &QD YHASI: COfiWOLLER SEPARABLE BX DECO~:BIIIATIOIi ?

A%monophasic culture of (i):e,n,x type obtained from 'S&. abortus-eaui (a):
e,n,x -x &J. tvohimurium i:l,2' was transferred to anti-e,n,x serum NGA deep
tube. h:(e,n,x) type of monopi;asic culture was selected from the deep tube.
In this monophasic type, H2 is expected to be stabilized in inactive state.
Consequently, the following transduction experiment will produce swarm only
when recombination occurred between H2 and phase controller.
Zk&. ty-ohimurium i:u --x the &:(enx) type ---$------+ (i):e.n,x or none ?
                           anti-i, a-1,2
                                  IYGA.

  Experiment.
A lysate of:'&&. t.mhi.murium !@42 (titer 4.5 x log/ml) was mixed with equal
volume of penassay broth culture of the k:(enx) type and the mixture was droped
on anti-i,l,2 NGA deep tubes, 0.1 ml in each.  So far, twenty five deep tube

cultures have been tested.  None of them produced swarm.  That is, no evidence
of recombination between H2 and phase controller has been obtained.