| |
Figure. Results of quantitative polymerase chain reaction (PCR) of
viral genome copies within organ samples taken from 2 Daubenton's bats infected
with European bat lyssavirus type 2 (EBLV-2). A) Standard curve of duplicate
dilutions of known quantities of EBLV-2 amplicon. A 20% (vol/vol) sample (10-fold
dilution series) separated by electrophoresis on a 1% agarose gel is shown in
the inset. Ct, threshold value. B) Real-time PCR amplification of EBLV-2
genomic RNA from organ samples from a bat (696/04) in Lancashire, United
Kingdom infected with EBLV-2. dR, fluorescence change generated by a
baseline-corrected algorithm calculated from every amplification cycle. A 20%
(vol/vol) sample of each amplification separated by electrophoresis on a 1%
agarose gel is shown in the inset. Br, brain; To, tongue; Li, liver; Ki,
kidney; In, intestine; St, stomach; -, negative sample (no template); +, positive
sample from a previous human case (5).
|
