Volume 10, Number 10, October 2004

Strain Typing Methods and Molecular Epidemiology of Pneumocystis Pneumonia

Charles Ben Beard,* Patricia Roux,† Gilles Nevez,‡ Philippe M. Hauser,§ Joseph A. Kovacs,¶ Thomas R. Unnasch,# and Bettina Lundgren**
*Centers for Disease Control and Prevention, Fort Collins, Colorado, USA; †Saint-Antoine University Hospital, Paris, France; ‡University of Picardy, Amiens, France; §University Hospital of Lausanne, Lausanne, Switzerland; ¶National Institutes of Health, Bethesda, Maryland, USA; #University of Alabama at Birmingham, Birmingham, Alabama, USA; and **Hvidovre Hospital, Hvidovre, Denmark

Figure 1. Schematic representation of the single-strand conformation polymorphism (SSCP) patterns of four variable regions used to type Pneumocystis jirovecii. Each lane corresponds to a hypothetical sample. All simple patterns with two bands for each region are shown. Each uppercase letter represents a simple SSCP pattern. For each region, the complex SSCP pattern A,B corresponding to the superimposition of simple patterns A and B is represented. The complex ITS1 pattern A,B, is demonstrated, in which pattern A is more abundant than pattern B. Reprinted with permission from reference 32. Hauser et al. 2001, AIDS:15(4):461–466.

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This page last reviewed September 21, 2004

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention