U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R81
Tris-Buffered Saline (TBS) (pH 7.5)

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Tris 2.42 g
NaCl 29.24 g
Double distilled water to make 1 liter

Dissolve ingredients. Adjust pH to 7.5 with HCl and bring volume to 1 liter.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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